Sánchez-Bonet Desirée, Padrón-Sanz Carolina, Lloris-Cejalvo José Miguel, Lloris-Carsí José Miguel, Cejalvo-Lapeña Dolores
Escuela de Doctorado, Universidad Católica de Valencia "San Vicente Mártir", Plaza de San Agustín, 3, 46001 Valencia, Spain.
Centro de Investigación Traslacional San Alberto Magno, Universidad Católica de Valencia "San Vicente Mártir", C/Quevedo, 2, 46001 Valencia, Spain.
Antioxidants (Basel). 2025 May 23;14(6):624. doi: 10.3390/antiox14060624.
is a brown seaweed known for its strong antioxidant properties, mainly attributed to its high polyphenolic content. This study aimed to evaluate the antioxidant protective effect of an optimised extract in an experimental model of intestinal ischemia/reperfusion (I/R) injury, considering the intestine as particularly vulnerable to this pathology. Seventy-two male Wistar albino rats were randomly divided into twelve groups: Sham, I/R groups (3 and 24 h reperfusion), I/R plus vehicle groups (three application times, 3 h reperfusion), and I/R plus extract groups (three application times, 3 and 24 h reperfusion). Intestinal injury was assessed through biochemical markers (malondialdehyde [MDA], superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx], and mieloperoxidase [MPO]), inflammatory cytokines (interleukin 1 β [IL-1β] and interleukin [IL-10]), and histological analysis. Results demonstrated that treatment with significantly reduced oxidative stress and inflammation caused by I/R injury ( < 0.05), restoring analysed parameters (MDA, SOD, CAT, IL-10) to levels comparable to the Sham group. Histological examination confirmed the preservation of intestinal mucosal integrity following administration. These findings suggest that the antioxidant extract from effectively protects against intestinal I/R injury, highlighting its potential for clinical use in preventing and managing this pathological condition, particularly in surgical contexts.
是一种棕色海藻，以其强大的抗氧化特性而闻名，这主要归因于其高多酚含量。本研究旨在评估一种优化提取物在肠缺血/再灌注（I/R）损伤实验模型中的抗氧化保护作用，考虑到肠道对这种病理状况特别敏感。72只雄性Wistar白化大鼠被随机分为12组：假手术组、I/R组（再灌注3小时和24小时）、I/R加赋形剂组（三个给药时间点，再灌注3小时）以及I/R加提取物组（三个给药时间点，再灌注3小时和24小时）。通过生化指标（丙二醛[MDA]、超氧化物歧化酶[SOD]、过氧化氢酶[CAT]、谷胱甘肽过氧化物酶[GPx]和髓过氧化物酶[MPO]）、炎性细胞因子（白细胞介素1β[IL-1β]和白细胞介素[IL-10]）以及组织学分析来评估肠道损伤。结果表明，提取物治疗显著降低了I/R损伤引起的氧化应激和炎症（P<0.05），使分析参数（MDA、SOD、CAT、IL-10）恢复到与假手术组相当的水平。组织学检查证实给药后肠道黏膜完整性得以保留。这些发现表明，该海藻的抗氧化提取物能有效保护肠道免受I/R损伤，突出了其在预防和管理这种病理状况，特别是在外科手术环境中的临床应用潜力。
