Köhler Simone, Wojcik Michal, Xu Ke, Dernburg Abby F
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA.
Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA.
Sci Adv. 2025 Jan 24;11(4):eadq9374. doi: 10.1126/sciadv.adq9374. Epub 2025 Jan 22.
During meiosis, pairing between homologous chromosomes is stabilized by the assembly of the synaptonemal complex (SC). The SC ensures the formation of crossovers between homologous chromosomes and regulates their distribution. However, how the SC regulates crossover formation remains elusive. We isolated an unusual mutation in that disrupts crossover interference but not SC assembly. This mutation alters the unique C terminal domain of an essential SC protein, SYP-4, a likely ortholog of the vertebrate SC protein SIX6OS1. We use three-dimensional stochastic optical reconstruction microscopy (3D-STORM) to interrogate the molecular architecture of the SC from wild-type and mutant animals. Using a probabilistic mapping approach to analyze super-resolution image data, we detect changes in the organization of the synaptonemal complex in wild-type animals that coincide with crossover designation. We also found that our mutant perturbs SC architecture. Our findings add to growing evidence that the SC is an active material whose molecular organization contributes to chromosome-wide crossover regulation.
在减数分裂过程中，同源染色体之间的配对通过联会复合体（SC）的组装得以稳定。SC确保同源染色体之间交叉互换的形成并调节其分布。然而，SC如何调节交叉互换的形成仍不清楚。我们在[物种名称]中分离出一种不寻常的突变，该突变破坏了交叉互换干扰，但不影响SC的组装。此突变改变了一种必需的SC蛋白SYP-4独特的C末端结构域，SYP-4可能是脊椎动物SC蛋白SIX6OS1的直系同源物。我们使用三维随机光学重建显微镜（3D-STORM）来研究野生型和突变型[物种名称]动物中SC的分子结构。通过概率映射方法分析超分辨率图像数据，我们检测到野生型动物中联会复合体组织的变化与交叉互换指定相吻合。我们还发现我们的[突变体名称]突变体扰乱了SC结构。我们的研究结果进一步证明，SC是一种活性物质，其分子组织有助于全染色体范围的交叉互换调节。
