用于确定禽血友病生长因子需求的市售培养盘的评估。

An evaluation of commercial discs for the determination of the growth factor requirements of avian haemophili.

作者信息

Blackall P J, Farrah J G

出版信息

Vet Microbiol. 1985 Jan;10(2):125-31. doi: 10.1016/0378-1135(85)90014-8.

Abstract

The ability of 2 brands of growth factor discs (Oxoid and Mast) to identify correctly the growth factor requirements of 41 isolates of Haemophilus paragallinarum and 17 isolates of H. avium was evaluated. The percentage of isolates correctly identified as requiring V factor varied with both the brand of the disc and the medium used. On basic nutrient media both brands gave low percentages of correct results: on Isosensitest Agar (Oxoid) the Oxoid discs gave 26% and the Mast discs 24% while on Heart Infusion Agar (Gibco) the Oxoid discs gave 54% and the Mast discs 28%. However, on a more complex medium (TM/S) the percentage of correct results was considerably higher with the Oxoid discs 100% and the Mast discs 52% accurate.

摘要

评估了2个品牌的生长因子圆盘（Oxoid和Mast）正确鉴定41株副鸡嗜血杆菌和17株鸟嗜血杆菌生长因子需求的能力。被正确鉴定为需要V因子的分离株百分比因圆盘品牌和所用培养基而异。在基本营养培养基上，两个品牌得出的正确结果百分比都很低：在伊索森测试琼脂（Oxoid）上，Oxoid圆盘得出的正确结果为26%，Mast圆盘为24%；而在心脏浸液琼脂（Gibco）上，Oxoid圆盘得出的正确结果为54%，Mast圆盘为28%。然而，在一种更复杂的培养基（TM/S）上，Oxoid圆盘得出的正确结果百分比要高得多，准确率为100%，Mast圆盘为52%。