Sueishi T, Hayashi Y, Iritani Y
Avian Dis. 1982 Jan-Mar;26(1):186-90.
Some solid media for antigen preparation of Haemophilus paragallinarum (HPG) hemagglutinin (HA) were studied. When HA activities of HPG strain 221 cultivated on chicken meat infusion (CMI) and gonococcal (GC) agar media were compared, the titer of the GC agar medium appeared faster and was higher than the titer on the CMI agar medium. For both agar media, the HA titer and growth increased with the concentration of the chicken serum added. In addition, HA activity on GC agar medium showed a higher titer than activity on other media, such as brain-heart infusion, Trypticase soy, blood, and Kato's agar media. When various HPG strains were inoculated onto GC agar medium, HA titers in strains 0083 and F were equal to that in strain 221. These results confirm that the GC agar medium is useful for preparation of HA antigen of HPG.
对一些用于副鸡嗜血杆菌（HPG）血凝素（HA）抗原制备的固体培养基进行了研究。比较了在鸡肉浸液（CMI）和淋球菌（GC）琼脂培养基上培养的HPG 221菌株的HA活性，发现GC琼脂培养基上的效价出现得更快且高于CMI琼脂培养基上的效价。对于这两种琼脂培养基，HA效价和生长均随添加的鸡血清浓度增加而升高。此外，GC琼脂培养基上的HA活性显示出比其他培养基（如脑心浸液、胰蛋白酶大豆、血液和加藤琼脂培养基）上的活性更高的效价。当将各种HPG菌株接种到GC琼脂培养基上时，0083和F菌株中的HA效价与221菌株中的相等。这些结果证实GC琼脂培养基可用于制备HPG的HA抗原。
