Wang Huan, Dong Lihua, Zhao Lijia, Sun Yu, Zhang Rui, Shan Guiye
Centre for Advanced Optoelectronic Functional Materials Research and Key Laboratory of UV Light-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, 130024, Changchun, China.
The Affiliated Hospital of Changchun University of Chinese Medicine, 130021, Changchun, China.
Biosens Bioelectron. 2025 Apr 1;273:117162. doi: 10.1016/j.bios.2025.117162. Epub 2025 Jan 13.
The development of integrated multiple signal outputs within a single platform is highly significant for efficient and accurate on-site biomarker detection. Herein, colorimetric/electrochemical dual-mode microfluidic paper-based analytical devices (μPADs) were designed for portable, visual and accurate dopamine (DA) detection. The dual-mode μPADs, featuring folded structure, integrate a colorimetric layer and an electrochemical layer using wax printing and laser-induced graphene (LIG) pyrolysis techniques, allowing the vertical flow of analyte solution. Copper sulfide @ silver sulfide (CuS@AgS) nanocomposites, with excellent laccase-like and electrocatalytic performance, were modified onto the detection zones as colorimetric/electrochemical sensing substrates for dual-mode DA recognition. In the colorimetric detection zones, the obvious violet-colored signals were generated based on the enhanced laccase-like oxidation reaction induced. Further, amplified electrical response signals toward DA were collected in electrochemical detection zone due to the epitaxial heterostructure of silver sulfide (AgS). Consequently, the developed CuS@AgS-incorporated μPADs exhibit linear responses for colorimetric and electrochemical DA detection in ranges of 2-50 μM and 0.5-70 μM, respectively, with the limit of detection (LOD) of 0.675 μM and 0.086 μM. This work integrates multiple detection modes into μPADs, providing the portable, reliable and visual alternative to traditional detection methods of DA for POCT.
在单个平台内开发集成的多信号输出对于高效、准确的现场生物标志物检测具有重要意义。在此，设计了比色/电化学双模式微流控纸基分析装置（μPADs）用于便携式、可视化且准确的多巴胺（DA）检测。具有折叠结构的双模式μPADs利用蜡印和激光诱导石墨烯（LIG）热解技术集成了比色层和电化学层，使分析物溶液能够垂直流动。具有优异类漆酶和电催化性能的硫化铜@硫化银（CuS@AgS）纳米复合材料被修饰到检测区域，作为用于双模式DA识别的比色/电化学传感底物。在比色检测区域，基于诱导的增强类漆酶氧化反应产生明显的紫色信号。此外，由于硫化银（AgS）的外延异质结构，在电化学检测区域收集到对DA放大的电响应信号。因此，所开发的包含CuS@AgS的μPADs在比色和电化学DA检测中分别在2 - 50 μM和0.5 - 70 μM范围内呈现线性响应，检测限（LOD）分别为0.675 μM和0.086 μM。这项工作将多种检测模式集成到μPADs中，为POCT中DA的传统检测方法提供了便携式、可靠且可视化的替代方案。
