Wang Huan, Dong Lihua, Ma Gina, Qiu Shuang, Shan Guiye, Zhao Lijia, Sun Yu, Cui Anni, Zhang Rui, Liu Xiaojie
Centre for Advanced Optoelectronic Functional Materials Research and Key Laboratory of UV Light-Emitting Materials and Technology of Ministry of Education, Northeast Normal University, Changchun, 130024, China.
The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, 130021, China.
Anal Chim Acta. 2025 Jan 22;1336:343489. doi: 10.1016/j.aca.2024.343489. Epub 2024 Nov 26.
Adrenaline and glucose are essential biomarkers in human body for maintaining metabolic balance. Abnormal levels of adrenaline and glucose are associated with various diseases. Therefore, it is important to design portable, on-site devices for rapid adrenaline and glucose analysis to safeguard health. Traditional paper-based analytical devices (μPADs) for multiplexed detection typically required multiple recognized probes, increasing the cost and complexity. Moreover, non-uniform color distribution, caused by lateral chromatographic elution effect, reduced results accuracy. Hence, it is necessary to construct visualized μPADs with controlled flow capability using single sensing probe for simultaneous adrenaline and glucose analysis in point-of-care testing (POCT).
The portable multiplexed 3D-folded μPADs have been developed for the visualized, accurate and simultaneous detection of adrenaline and glucose, employing Ag-doped copper sulfide nanoparticles (CuAgS NPs) as multifunctional sensing probe. The μPADs integrated the sampling entrance layer, buffer layer and test layer into a single device through wax printing, allowing the analyte solution to flow vertically from buffer zones to detection zones, thereby minimizing lateral chromatographic elution effect. CuAgS NPs, exhibiting laccase-like and peroxidase-like multi-enzyme activities, were modified on various detection zones of μPADs for multiplex colorimetric analysis of adrenaline and glucose. Based on CuAgS-induced colorimetric reactions, the orange-red and blue colors were generated in adrenaline and glucose detection zones, respectively. Subsequently, color signals were converted into RGB values via smartphone application (APP) for rapid and intelligent results analysis. The CuAgS-incorporated μPADs exhibited colorimetric uniformity and accuracy with limit of detection (LOD) of 10.2 nM and 11.5 μM, respectively.
The adjustable laccase-like and peroxidase-like activities of CuAgS NPs induced by Ag doping provide a new perspective for multiple biomarkers analysis. Benefiting from the excellent multienzyme activity of CuAgS NPs, μPADs can perform on-site, visual and multiplex analysis using a single probe, demonstrating their potential for POCT in personal healthcare. The 3D folded CuAgS-incorporated μPADs with integrated horizontal and vertical flows exhibit uniform color signals for obtaining accurate detection results.
肾上腺素和葡萄糖是人体维持代谢平衡的重要生物标志物。肾上腺素和葡萄糖水平异常与多种疾病相关。因此，设计便携式现场设备用于快速分析肾上腺素和葡萄糖以保障健康非常重要。传统的用于多重检测的纸质分析设备（μPADs）通常需要多个识别探针，这增加了成本和复杂性。此外，横向色谱洗脱效应导致的颜色分布不均匀降低了结果的准确性。因此，有必要构建具有可控流动能力的可视化μPADs，使用单一传感探针在即时检测（POCT）中同时分析肾上腺素和葡萄糖。
已开发出便携式多重三维折叠μPADs，用于可视化、准确且同时检测肾上腺素和葡萄糖，采用掺银硫化铜纳米颗粒（CuAgS NPs）作为多功能传感探针。μPADs通过蜡印将采样入口层、缓冲层和测试层集成到单个设备中，使分析物溶液从缓冲区域垂直流向检测区域，从而最大限度地减少横向色谱洗脱效应。具有类似漆酶和过氧化物酶多酶活性的CuAgS NPs被修饰在μPADs的各个检测区域，用于肾上腺素和葡萄糖的多重比色分析。基于CuAgS诱导的比色反应，在肾上腺素和葡萄糖检测区域分别产生橙红色和蓝色。随后，通过智能手机应用程序（APP）将颜色信号转换为RGB值，以进行快速和智能的结果分析。掺入CuAgS的μPADs表现出比色均匀性和准确性，检测限（LOD）分别为10.2 nM和11.5 μM。
银掺杂诱导的CuAgS NPs的可调类似漆酶和过氧化物酶活性为多种生物标志物分析提供了新视角。受益于CuAgS NPs出色的多酶活性，μPADs可以使用单个探针进行现场、可视化和多重分析，展示了它们在个人医疗保健POCT中的潜力。具有集成水平和垂直流的三维折叠掺入CuAgS的μPADs表现出均匀的颜色信号，以获得准确的检测结果。
