de Souza Amanda, Amaral Gustavo Oliva, do Espirito Santo Giovanna, Dos Santos Jorge Sousa Karolyne, Martignago Cintia Cristina Santi, Souza E Silva Lais Caroline, de Lima Lindiane Eloisa, Vitor de Souza Daniel, Cruz Matheus Almeida, Ribeiro Daniel Araki, Granito Renata Neves, Renno Ana Claudia Muniz
Department of Biosciences, Federal University of São Paulo (UNIFESP), 136 Silva Jardim Street, 11015020 Santos, SP, Brazil.
Biomed Mater. 2025 Jan 31;20(2). doi: 10.1088/1748-605X/adad29.
The search for innovative materials for manufacturing skin dressings is constant and high demand. In this context, the present study investigated the effects of a 3D printed skin dressing made of spongin-like collagen (SC) extract from marine sponge (), used in 3 concentrations of SC and alginate (C1, C2, C3). For this proposal, the physicochemical, morphological andbiological results were investigated. The results demonstrated that, after immersion, C2 presented a higher mass loss and C3 present a higher pH in experimental periods. Also, a higher porosity was observed for C1 and C2 skin dressings, with a higher swelling ratio for C2. For Fourier transform infrared, peaks of Amide A, -CH2, -COOH and C-O-C were seen. Moreover, the macroscopic image demonstrated a skin dressing with rough surface and grayish color that is naturally observed in. For scanning electron microscopy analysis the presence of pores could be observed for all skin dressings, with fibers disposed in layers. Theanalyses demonstrated the viability of HFF-1 and L929 cell lines 70% of the values found for cell proliferation compared to Control Group. Furthermore, the cell adhesion analysis demonstrated that both cell lines adhered to the 3 different skin dressings and non-cytotoxicity was observed. Taking together, all the results suggest that the skin dressings are biocompatible and present non-cytotoxicity in thestudies, being considered a suitable material for tissue engineering proposals.
寻找用于制造皮肤敷料的创新材料的工作一直在持续且需求旺盛。在此背景下，本研究调查了由海洋海绵中提取的类海绵胶原蛋白（SC）制成的3D打印皮肤敷料的效果，使用了3种浓度的SC和藻酸盐（C1、C2、C3）。针对此方案，研究了其物理化学、形态学和生物学结果。结果表明，浸泡后，在实验期间C2的质量损失更高，C3的pH值更高。此外，观察到C1和C2皮肤敷料的孔隙率更高，C2的膨胀率更高。对于傅里叶变换红外光谱，观察到了酰胺A、-CH2、-COOH和C-O-C的峰。此外，宏观图像显示出一种表面粗糙、颜色呈灰色的皮肤敷料，这在……中是自然观察到的。对于扫描电子显微镜分析，所有皮肤敷料都能观察到孔隙的存在，纤维呈层状排列。分析表明，与对照组相比，HFF-1和L929细胞系的活力为细胞增殖值的70%。此外，细胞黏附分析表明，两种细胞系都能黏附在3种不同的皮肤敷料上，且未观察到细胞毒性。综上所述，所有结果表明皮肤敷料具有生物相容性，在研究中表现出无细胞毒性，被认为是组织工程方案的合适材料。
