Friesen A D, Bowman J M, Bees W C
Vox Sang. 1985;48(4):201-12. doi: 10.1111/j.1423-0410.1985.tb00173.x.
Column ion exchange chromatographic processes were developed for the production of immune serum globulin (ISG) for intravenous use. Sequential chromatography on two cross-linked agarose gel anion exchangers yielded the same immunoelectrophoretic pure ISG as a single column DEAE-Sephadex procedure with the advantage of repeated 'in column' cycling. ISG with a low content of aggregated IgG and low anticomplementary activity was prepared from plasma with 53-69% efficiency. The ISG prepared by these processes was characterized with respect to the content of IgA, prekallikrein activator (PKA), plasminogen, fragmentation, and heavy chain subclass distribution.
开发了柱离子交换色谱法来生产静脉注射用免疫血清球蛋白（ISG）。在两种交联琼脂糖凝胶阴离子交换剂上进行连续色谱法，得到的免疫电泳纯ISG与单柱DEAE-葡聚糖凝胶法相同，具有重复“柱内”循环的优点。从血浆中制备了聚集IgG含量低且抗补体活性低的ISG，效率为53-69%。通过这些方法制备的ISG在IgA、前激肽释放酶激活剂（PKA）、纤溶酶原、片段化和重链亚类分布方面进行了表征。
