Zhang Kai, Yue Lei, Cong Jingxian, Zhang Jianlong, Feng Zhibin, Yang Qian, Lu Xuechun
School of Life Science, Ludong University, 186 Hongqi Road, Yantai, Shandong 264025, China; School of Life Science and Technology, Harbin Institute of Technology, Harbin 150006, China.
School of Life Science, Ludong University, 186 Hongqi Road, Yantai, Shandong 264025, China.
Carbohydr Polym. 2025 Mar 15;352:123196. doi: 10.1016/j.carbpol.2024.123196. Epub 2024 Dec 28.
Environmental pH is an important parameter that impacts the growth, reproduction, and carbohydrate metabolism of Aureobasidium spp.. This study identifies the ApGph1 gene (encoded with Glycogen Phosphatase) reflecting significant carbohydrate metabolism difference through transcriptome analysis of Aureobasidium Pullulans YQ65 cultured under different pH. It is subsequently analyzed using the Conserved Domains and Expasy tools. It has been found that compared with its wild type, the △ApGph1 strain exhibits no significant differences in its growth pattern and morphology but a production volume of pullulan inversely proportional to its glycogen content. In addition, through fed-batch fermentation, an over-expressed ApGph1 strain can produce 42.7 g/L of pullulan within 144 h, which is related to the increased expression of key genes involved in pullulan synthesis. The results can provide a guide for the industrial production of pullulan.
环境pH是影响金担子菌属生长、繁殖和碳水化合物代谢的重要参数。本研究通过对在不同pH条件下培养的出芽短梗霉YQ65进行转录组分析，鉴定出反映显著碳水化合物代谢差异的ApGph1基因（由糖原磷酸酶编码）。随后使用保守结构域和Expasy工具对其进行分析。研究发现，与野生型相比，△ApGph1菌株在生长模式和形态上没有显著差异，但普鲁兰多糖的产量与其糖原含量成反比。此外，通过分批补料发酵，过表达ApGph1的菌株在144小时内可产生42.7 g/L的普鲁兰多糖，这与参与普鲁兰多糖合成的关键基因表达增加有关。该结果可为普鲁兰多糖的工业化生产提供指导。
