Saab Marc, Drucbert Anne-Sophie, Blanchemain Nicolas, Chai Feng
Service d'Orthopédie 1 et Traumatologie, Hôpital Roger Salengro, Lille, F-59000, France.
U1008 - Advanced Drug Delivery Systems and Biomaterials, Univ. Lille, INSERM, CHU Lille, Lille, F-59000, France.
Sci Rep. 2025 Jan 22;15(1):2796. doi: 10.1038/s41598-025-85607-w.
This study aimed to compare the failure rates of two different sizes of plates and screws to stabilize critical-sized (7 mm) femoral defects in male Sprague‒Dawley rats (aged 10 weeks). Femoral defects were stabilized with either a 4-hole plate (length 29 mm, thickness 1 mm, 10 rats, Group 1) and 4 cortical screws (diameter 2 mm) or with a 6-hole plate (length 30 mm, thickness 0.6 mm, 9 rats, Group 2) and 4 cortical screws (diameter 1.5 mm). A polymethylmethacrylate spacer was inserted into the defects to reproduce the first stage of the induced membrane technique. Radiographic evaluations, macroscopic and histologic assessments of the induced membranes were conducted at 1 week and 4 weeks. No implant failure occurred in Group 1 whereas in Group 2, 4/9 (44.4%) implant failures occurred during the follow-up (p = 0.03). On histomorphometry, cell density was higher in Group 1 (4996 ± 716 cells / mm²) than in Group 2 (3500 ± 728 cells/mm²) (p = 0.0195) but the membrane thickness in Group 1 (735 ± 44 μm) was non-significantly lower than in Group 2 (979 ± 165 μm) (p = 0.4). This study suggests that, in rat models of critical-sized femoral defects (7 mm) to study the induced membrane technique, fixation plates with a thickness of 1 mm and four screws (2 mm in diameter) provide stable fixation without implant failure. In contrast, thinner plates (< 1 mm) combined with screws of smaller diameter (1.5 mm) result in a high rate of implant failure.
本研究旨在比较两种不同尺寸的接骨板和螺钉在稳定10周龄雄性斯普拉格-道利大鼠临界尺寸（7毫米）股骨缺损中的失败率。股骨缺损分别用4孔接骨板（长度29毫米，厚度1毫米，10只大鼠，第1组）和4枚皮质骨螺钉（直径2毫米），或用6孔接骨板（长度30毫米，厚度0.6毫米，9只大鼠，第2组）和4枚皮质骨螺钉（直径1.5毫米）进行固定。将聚甲基丙烯酸甲酯间隔物插入缺损处以重现诱导膜技术的第一阶段。在1周和4周时对接骨板进行影像学评估、对诱导膜进行大体和组织学评估。第1组未发生植入物失败，而在第2组中，随访期间有4/9（44.4%）发生植入物失败（p = 0.03）。组织形态计量学显示，第1组的细胞密度（4996±716个细胞/mm²）高于第2组（3500±728个细胞/mm²）（p = 0.0195），但第1组的膜厚度（735±44μm）略低于第2组（979±165μm），差异无统计学意义（p = 0.4）。本研究表明，在用于研究诱导膜技术的临界尺寸（7毫米）股骨缺损大鼠模型中，厚度为1毫米的接骨板和4枚螺钉（直径2毫米）可提供稳定固定，不会发生植入物失败。相比之下，较薄的接骨板（<1毫米）与较小直径的螺钉（1.5毫米）结合会导致较高的植入物失败率。
