Laczkó Levente, Nagy Nikoletta Andrea, Nagy Ágnes, Maroda Ágnes, Sály Péter
One Health Institute, University of Debrecen, Debrecen, Hungary.
HUN-REN-UD Conservation Biology Research Group, University of Debrecen, Debrecen, Hungary.
Sci Data. 2025 Jan 22;12(1):137. doi: 10.1038/s41597-025-04469-z.
The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout Europe, primarily inhabiting stony upper sections of stream networks. This study presents an updated genome assembly of B. barbatula, contributing to the species' available genomic resources for downstream applications such as conservation genetics. The draft assembly was 550 Mbp in size, with an N50 of 11.21 Mbp. We used the species' available chromosome scaffolds to finish the genome. The final assembly had a BUSCO score of 96.7%. We identified 23270 protein-coding genes, and the proteome exhibited high completeness with BUSCO (93.1%) and OMArk (90.81%). Despite using multiple approaches to reduce duplicate contigs, we observed a relatively high duplicate ratio of 6.1% (BUSCO) and 8.52% (OMArk) in the annotations. We aimed to find microsatellite loci present in both the species' publicly available genome and the new assembly to aid marker development for downstream analyses. This dataset serves as a reference for genomic analysis and is useful for developing markers to study the species' biodiversity and support conservation efforts.
石爬鮡（Barbatula barbatula）是一种底栖鱼类，广泛分布于欧洲各地，主要栖息在河网的多石上游区域。本研究展示了石爬鮡的一个更新的基因组组装结果，为该物种的基因组资源增添了内容，可用于诸如保护遗传学等下游应用。该草图组装大小为550兆碱基对，N50为11.21兆碱基对。我们利用该物种现有的染色体支架完成了基因组组装。最终组装的BUSCO得分是96.7%。我们鉴定出23270个蛋白质编码基因，蛋白质组在BUSCO（93.1%）和OMArk（90.81%）评估中显示出高度完整性。尽管使用了多种方法来减少重复重叠群，但我们在注释中观察到相对较高的重复率，BUSCO为6.1%，OMArk为8.52%。我们旨在找到该物种公开可用基因组和新组装中都存在的微卫星位点，以辅助开发用于下游分析的标记。该数据集可作为基因组分析的参考，有助于开发标记来研究该物种的生物多样性并支持保护工作。
