Salehi Farid Ali, Rowley Jennifer E, Allen Harris H, Kruger Isabella G, Tavakolpour Soheil, Neeley Kyle, Cong Min, Shahbazian Haneyeh, Dorafshani Niki, Berrada Achraf, MacDonagh Alexander C, Padera Robert F, Brugarolas Pedro, Packard Alan B, Rosenbaum Matthew W, Divakaran Sanjay, Di Carli Marcelo F, Rashidian Mohammad
Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
Nature. 2025 Mar;639(8053):214-224. doi: 10.1038/s41586-024-08441-6. Epub 2025 Jan 22.
Imaging inflammation holds immense potential for advancing the diagnosis, treatment and prognosis of many conditions. The lack of a specific and sensitive positron emission tomography (PET) probe to detect inflammation is a critical challenge. To bridge this gap, we present CD45-PET imaging, which detects inflammation with exceptional sensitivity and clarity in several preclinical models. Notably, the intensity of the CD45-PET signal correlates robustly with the severity of disease in models of inflammatory lung and bowel diseases, outperforming F-fluorodeoxyglucose PET, the most widely used imaging modality for inflammation globally. Longitudinal CD45-PET imaging further enables precise monitoring of dynamic changes in tissue-specific inflammatory profiles. Finally, we developed a human CD45-PET probe for clinical translation that effectively detects human immune cells in a humanized mouse model. CD45-PET imaging holds substantial clinical promise, offering a tool for guiding diagnostic and therapeutic decisions for inflammatory diseases through a precise, whole-body assessment of the inflammation profiles of individual patients.
成像炎症在推进多种病症的诊断、治疗和预后方面具有巨大潜力。缺乏用于检测炎症的特异性和灵敏的正电子发射断层扫描（PET）探针是一项关键挑战。为弥补这一差距，我们展示了CD45-PET成像，其在多种临床前模型中以极高的灵敏度和清晰度检测炎症。值得注意的是，在炎症性肺病和肠病模型中，CD45-PET信号强度与疾病严重程度密切相关，优于全球最广泛使用的炎症成像方式——F-氟脱氧葡萄糖PET。纵向CD45-PET成像还能够精确监测组织特异性炎症特征的动态变化。最后，我们开发了一种用于临床转化的人CD45-PET探针，该探针可在人源化小鼠模型中有效检测人免疫细胞。CD45-PET成像具有重大的临床前景，通过对个体患者炎症特征进行精确的全身评估，为指导炎症性疾病的诊断和治疗决策提供了一种工具。
