Khalid Wajeeha, Aslam Afeefa, Ahmed Nadeem, Sarfraz Muhammad, Khan Jawad Akbar, Mohsin Sabeeh, Rajoka Muhammad Shahid Riaz, Nazir Imran, Amirzada Muhammad Imran
Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Abbottabad, 22060, Pakistan.
Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 54000, Pakistan.
AAPS PharmSciTech. 2025 Jan 22;26(1):42. doi: 10.1208/s12249-024-03028-w.
The aim of the present study was to investigate the potential of human plasma derived exosomes for the delivery of hydroxyurea to enhance its therapeutic efficacy in breast cancer. Plasma derived exosomes were isolated using differential centrifugation along with ultrafiltration method. Hydroxyurea was encapsulated in exosomes using a freeze-thaw method. The exosomes and Exo-HU were characterized for their size distribution, drug entrapment efficiency, in-vitro drug release profile, morphological analysis and cytotoxic effects on MCF-7 cell line. The results showed a mean size of 178.8 nm and a zeta potential of -18.3 mV, indicating good stability and 70% encapsulation effectiveness for HU. Exo-HU produced sustained drug release action with a considerable percentage released within 72 h. The morphological analysis indicated that the plasma derived exosomes were spherical, and cup shaped. In cytotoxicity studies on MCF-7 cells, Exo-HU has reduced cell viability compared to HU and blank exosomes. Findings of this study showed that human plasma-derived exosomes have been considered as effective delivery vehicle for hydroxyurea, potentially improving breast cancer treatment outcomes.
本研究的目的是探讨人血浆来源的外泌体用于递送羟基脲以增强其在乳腺癌治疗中的疗效的潜力。采用差速离心结合超滤法分离血浆来源的外泌体。使用冻融法将羟基脲包封在外泌体中。对外泌体和Exo-HU进行了大小分布、药物包封效率、体外药物释放曲线、形态分析以及对MCF-7细胞系的细胞毒性作用的表征。结果显示平均大小为178.8nm，zeta电位为-18.3mV，表明具有良好的稳定性，且羟基脲的包封效率为70%。Exo-HU产生持续的药物释放作用，在72小时内有相当比例的药物释放。形态分析表明血浆来源的外泌体呈球形和杯状。在对MCF-7细胞的细胞毒性研究中，与羟基脲和空白外泌体相比，Exo-HU降低了细胞活力。本研究结果表明，人血浆来源的外泌体被认为是羟基脲的有效递送载体，可能改善乳腺癌的治疗效果。
