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工程化巨噬细胞膜仿生载基因纳米球靶向给药促进深Ⅱ度烧伤创面修复的机制

Mechanism of engineered macrophage membrane bionic gene-carrying nanospheres for targeted drug delivery to promote wound repair in deep second-degree burns.

作者信息

Zhu Zhihan, Zhu Xinghua, Miao Shichen, Wang Bolin, Li Zihan, Zhang Dinghao, Zou Shentian, Zhang Yi, Zhang Qingrong, Hu Kesu

机构信息

Department of Burn and Plastic Surgery, Medical School of Nantong University, Nantong, 226000, Jiangsu, China.

Department of Burn and Plastic Surgery, The First People's Hospital of Changzhou, Jiangsu, 213000, China.

出版信息

Sci Rep. 2025 Jan 22;15(1):2756. doi: 10.1038/s41598-025-86716-2.

DOI:10.1038/s41598-025-86716-2
PMID:39843907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11754751/
Abstract

Hepatocyte growth factor (HGF) is a substance that stimulates the proliferation of hepatocytes which promote healing. We developed a macrophage membrane-encapsulated nanosphere drug delivery system containing HGF for the study of burn wound healing. Twenty-seven Sprague-Dawley rats were randomly divided into three groups: a saline control (NS) group, an engineered macrophage membrane-encapsulated nanospheres (ETMM@NPS) group, and an engineered macrophage membrane-encapsulated nanospheres treatment with HGF-loaded gene (HGF@ETMM@NPS) group.The wound tissue sections were examined histologically using hematoxylin and eosin (H&E) and Masson trichrome staining. Immunohistochemistry and Western blotting were performed to determine the expression of relevant proteins. The wound-healing, blood flow and complete epithelialization rates were significantly better in the HGF@ETMM@NPS group compared to the NS and ETMM@NPS groups. Expression of B-cell lymphoma 2-associated X-protein was significantly lower, and B-cell lymphoma 2, cluster of differentiation 31, HGF, alpha smooth muscle actin, and PCNA expression was significantly higher in the HGF@ETMM@NPS group compared with the other two groups. PCNA and HGF expression was significantly up-regulated in the HGF@ETMM@NPS group. The HGF@ETMM@NPS complex drug delivery system used in this research promoted wound healing via effective delivery of HGF to burn wounds, thereby accelerating skin cell growth and migration.

摘要

肝细胞生长因子（HGF）是一种刺激肝细胞增殖从而促进愈合的物质。我们开发了一种含有HGF的巨噬细胞膜包裹纳米球药物递送系统，用于研究烧伤创面愈合。将27只Sprague-Dawley大鼠随机分为三组：生理盐水对照组（NS）、工程化巨噬细胞膜包裹纳米球（ETMM@NPS）组和负载HGF基因的工程化巨噬细胞膜包裹纳米球治疗组（HGF@ETMM@NPS）。使用苏木精和伊红（H&E）染色及Masson三色染色对伤口组织切片进行组织学检查。进行免疫组织化学和蛋白质印迹分析以确定相关蛋白的表达。与NS组和ETMM@NPS组相比，HGF@ETMM@NPS组的伤口愈合、血流及完全上皮化率明显更好。与其他两组相比，HGF@ETMM@NPS组中B细胞淋巴瘤2相关X蛋白的表达明显降低，而B细胞淋巴瘤2、分化簇31、HGF、α平滑肌肌动蛋白和增殖细胞核抗原（PCNA）的表达明显升高。HGF@ETMM@NPS组中PCNA和HGF的表达明显上调。本研究中使用的HGF@ETMM@NPS复合药物递送系统通过将HGF有效递送至烧伤创面促进伤口愈合，从而加速皮肤细胞生长和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/4a66497257c2/41598_2025_86716_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/0903620da65e/41598_2025_86716_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/a8ec8886fdd8/41598_2025_86716_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/fe6f3b251e17/41598_2025_86716_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/0f87ff649856/41598_2025_86716_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/72c12910b7f4/41598_2025_86716_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/3a0429a85fbc/41598_2025_86716_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/b410125d93c3/41598_2025_86716_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/e19935fbb5c0/41598_2025_86716_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1eb/11754751/4a66497257c2/41598_2025_86716_Fig11_HTML.jpg

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