Dusanic D G
Z Parasitenkd. 1985;71(1):19-31. doi: 10.1007/BF00932915.
Adrenals, hearts, kidneys, livers, lungs, and spleens were removed from C3H/Anf mice which had been inoculated with Trypanosoma (Herpetosoma) musculi and no longer exhibited parasitemias. Imprints of each organ were examined microscopically, and each was homogenized and injected into recipient mice. It was confirmed that trypanosomes could be detected only in the donor kidneys. Lampit or Ethidium treatment eliminated bloodstream and kidney forms when administration was initiated after the development of patent parasitemias. However, mice treated with Lampit on the same day they were inoculated with T. musculi developed parasitemias later than animals injected with drug after parasites had appeared in their blood. Both Lampit and Ethidium depressed antibody production as detected in enzyme-linked immunosorbent assays of antisera from animals having parasitemias at the time of treatment. The elimination of kidney forms by Lampit or Ethidium treatment did not reduce the resistance of mice to reinfection by T. musculi 12 weeks or 15 and 22 weeks, respectively, after the initial inoculation of these animals with the parasites. Kidney forms were not required for the sustained protective immunity of the mice against reinfection during the intervals of these experiments.
从已接种克氏锥虫（肌爬虫亚属）且不再出现寄生虫血症的C3H/Anf小鼠身上取出肾上腺、心脏、肾脏、肝脏、肺和脾脏。对每个器官的压印片进行显微镜检查，并将每个器官匀浆后注射到受体小鼠体内。经证实，仅在供体肾脏中能检测到锥虫。在出现明显寄生虫血症后开始给药时，蓝胺嘧啶或乙锭治疗可消除血液和肾脏中的虫体。然而，在接种克氏锥虫当天用蓝胺嘧啶治疗的小鼠，其出现寄生虫血症的时间比在血液中出现寄生虫后注射药物的动物要晚。在酶联免疫吸附试验中，对治疗时患有寄生虫血症的动物的抗血清检测发现，蓝胺嘧啶和乙锭均会抑制抗体产生。在初次接种寄生虫后的12周、15周和22周，分别用蓝胺嘧啶或乙锭治疗消除肾脏中的虫体，并未降低小鼠对克氏锥虫再次感染的抵抗力。在这些实验期间，小鼠对再次感染的持续保护性免疫并不需要肾脏中的虫体。
