Suppr超能文献

特定化疗对恰加斯病中溶解抗体水平的影响。

Effect of specific chemotherapy on the levels of lytic antibodies in Chagas's disease.

作者信息

Krettli A U, Cançado J R, Brener Z

出版信息

Trans R Soc Trop Med Hyg. 1982;76(3):334-40. doi: 10.1016/0035-9203(82)90184-5.

Abstract

Clinical trials with compounds active in Chagas's disease have shown that after treatment parasitological diagnostic methods (xenodiagnosis) become repeatedly negative whereas conventional serology (immunofluorescence and complement fixation tests) persists steadily positive. Consequently, assessment of cure still remains controversial. This paper reports the influence of specific treatment on antibodies involved in the conventional serological diagnosis and on antibodies which bind to the living bloodstream forms and are related to host resistance. Antibodies lytic to Trypanosoma cruzi bloodstream stages were detected, through a complement-mediated lysis (CML) test, in: (a) 100% of 28 untreated patients; (b) 94% of a group of 21 treated patients in whom conventional serology remained positive, including those with persistently negative xenodiagnosis; (c) 0% of 17 normal controls. In some patients treated with a nitrofuran derivative (nifurtimox) or with a 2-nitroimidazole derivative (benznidazol), CML test became gradually negative whereas conventional serology continued to be positive. Finally, in five patients treated with benznidazol, serological tests, CML and xenodiagnosis became regularly negative, strongly suggesting parasitological cure. Those findings demonstrate a dissociation between the antibodies mediating serological diagnosis and those directed against living bloodstream parasites. Moreover, since in some patients both types of antibodies disappeared after treatment, the results suggest that cure of Chagas's disease should be based not only on negative xenodiagnosis but also on the elimination of specific antibodies detectable by conventional serology and CML test.

摘要

针对恰加斯病具有活性的化合物进行的临床试验表明,治疗后寄生虫学诊断方法(异种接种诊断)反复呈阴性,而传统血清学方法(免疫荧光和补体结合试验)却持续呈阳性。因此,关于治愈的评估仍存在争议。本文报告了特异性治疗对传统血清学诊断中涉及的抗体以及与活的血液内寄生虫结合且与宿主抵抗力相关的抗体的影响。通过补体介导的溶血(CML)试验,在以下人群中检测到对克氏锥虫血液内阶段具有溶细胞作用的抗体:(a)28例未治疗患者中的100%;(b)21例接受治疗但传统血清学仍呈阳性的患者中的94%,包括异种接种诊断持续呈阴性的患者;(c)17名正常对照者中的0%。在一些接受硝基呋喃衍生物(硝呋替莫)或2-硝基咪唑衍生物(苯硝唑)治疗的患者中,CML试验逐渐变为阴性,而传统血清学仍呈阳性。最后,在5例接受苯硝唑治疗的患者中,血清学试验、CML试验和异种接种诊断均定期变为阴性,强烈提示寄生虫学治愈。这些发现表明介导血清学诊断的抗体与针对活的血液内寄生虫的抗体之间存在分离。此外,由于在一些患者中,治疗后这两种抗体均消失,结果提示恰加斯病的治愈不仅应基于异种接种诊断呈阴性,还应基于消除传统血清学和CML试验可检测到的特异性抗体。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验