牛冠状病毒血凝素蛋白
Bovine coronavirus hemagglutinin protein.
作者信息
King B, Potts B J, Brian D A
出版信息
Virus Res. 1985 Feb;2(1):53-9. doi: 10.1016/0168-1702(85)90059-0.
Abstract
Treatment of purified bovine coronavirus (Mebus strain) with pronase destroyed the integrity of virion surface glycoproteins gp140, gp120, gp100, reduced the amount of gp26 and destroyed the hemagglutinating activity of the virus. Bromelain, on the other hand, destroyed the integrity of gp120, gp100 and gp26 but failed to remove gp140 and failed to destroy viral hemagglutinating activity. These experiments suggest that gp140 is the virion hemagglutinin. Immunoblotting studies using monospecific antiserum demonstrate that gp140 is a disulfide-linked dimeric structure reducible to monomers of 65 kDa.
摘要
用链霉蛋白酶处理纯化的牛冠状病毒（梅布斯毒株）会破坏病毒粒子表面糖蛋白gp140、gp120、gp100的完整性，减少gp26的量，并破坏病毒的血凝活性。另一方面，菠萝蛋白酶会破坏gp120、gp100和gp26的完整性，但无法去除gp140，也无法破坏病毒的血凝活性。这些实验表明gp140是病毒粒子血凝素。使用单特异性抗血清的免疫印迹研究表明，gp140是一种二硫键连接的二聚体结构，可还原为65 kDa的单体。
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