Hogue B G, King B, Brian D A
J Virol. 1984 Aug;51(2):384-8. doi: 10.1128/JVI.51.2.384-388.1984.
Antisera prepared against each of three single and one pair of major structural proteins of the bovine coronavirus (Mebus strain) were used in immunoblotting studies to measure cross-reactivity with the structural proteins of the human coronavirus OC43 and the mouse hepatitis coronavirus A59. We conclude that the bovine coronavirus is comprised of four major structural proteins, gp190 (normally present as 120- and 100-kilodalton subunits), gp140, pp52, and gp26. The human coronavirus OC43 has an antigenically homologous counterpart of similar molecular mass to each of these proteins. The mouse hepatitis coronavirus A59 has an antigenically homologous counterpart to only three of these proteins: gp190, pp52 and gp26. There is no counterpart in the mouse virus to the 140-kilodalton glycoprotein, the apparent hemagglutinin of the bovine coronavirus.
用针对牛冠状病毒（梅布斯毒株）三种单一主要结构蛋白和一对主要结构蛋白制备的抗血清进行免疫印迹研究，以检测其与人冠状病毒OC43和小鼠肝炎冠状病毒A59结构蛋白的交叉反应性。我们得出结论，牛冠状病毒由四种主要结构蛋白组成，即gp190（通常以120千道尔顿和100千道尔顿亚基形式存在）、gp140、pp52和gp26。人冠状病毒OC43对这些蛋白中的每一种都有分子质量相似的抗原同源对应物。小鼠肝炎冠状病毒A59仅对其中三种蛋白有抗原同源对应物：gp190、pp52和gp26。小鼠病毒中没有与牛冠状病毒明显的血凝素——140千道尔顿糖蛋白相对应的蛋白。
