Cariati Ida, Bonanni Roberto, Romagnoli Cristian, Caprioli Lucio, D'Arcangelo Giovanna, Tancredi Virginia, Annino Giuseppe
Department of Systems Medicine, "Tor Vergata" University of Rome, 00133 Rome, Italy.
Department of Biomedicine and Prevention, "Tor Vergata" University of Rome, 00133 Rome, Italy.
J Funct Morphol Kinesiol. 2025 Jan 10;10(1):26. doi: 10.3390/jfmk10010026.
: Whole body vibration (WBV) is a valuable tool to mitigate physiological adaptations related to age and inactivity. Although significant benefits have been found at the musculoskeletal level, including increased bone mass and reduced muscle atrophy, the underlying biological mechanisms remain largely unknown. Therefore, our study aimed to evaluate the effects of vibratory training on bone tissue in murine models of different age groups by investigating the structural and distribution changes in some crucial biomarkers involved in musculoskeletal homeostasis. : Specifically, 4-, 12-, and 24-month-old mice were trained with a WBV protocol characterized by three series of 2 min and 30 s, interspersed with a recovery period of the same duration, on a 3-weekly frequency for 3 months. At the end of the training, histological and morphometric analyses were conducted, in association with immunohistochemical analysis to investigate changes in the distribution of fibronectin type III domain-containing protein 5 (FNDC5), NADPH oxidase 4 (NOX4), and sirtuin 1 (SIRT1). : Our preliminary results showed that WBV improves musculoskeletal health by preserving bone architecture and promoting up-regulation of FNDC5 and SIRT1 and down-regulation of NOX4. : Our study confirms vibratory training as a viable alternative to counter musculoskeletal decline in elderly and/or sedentary subjects. Further investigations should be conducted to deepen knowledge in this field and explore the role of other molecular mediators in physiological adaptations to vibration.
全身振动（WBV）是一种减轻与年龄和缺乏运动相关的生理适应性变化的有效工具。尽管在肌肉骨骼层面已发现显著益处，包括增加骨量和减少肌肉萎缩，但其潜在的生物学机制在很大程度上仍不为人知。因此，我们的研究旨在通过研究参与肌肉骨骼内稳态的一些关键生物标志物的结构和分布变化，评估振动训练对不同年龄组小鼠模型骨组织的影响。具体而言，对4个月、12个月和24个月大的小鼠进行全身振动训练，训练方案为3组，每组2分30秒，组间穿插相同时长的恢复期，每周3次，持续3个月。训练结束时，进行组织学和形态计量学分析，并结合免疫组织化学分析，以研究含III型纤连蛋白结构域蛋白5（FNDC5）、NADPH氧化酶4（NOX4）和沉默调节蛋白1（SIRT1）的分布变化。我们的初步结果表明，全身振动通过保持骨骼结构、促进FNDC5和SIRT1的上调以及NOX4的下调来改善肌肉骨骼健康。我们的研究证实，振动训练是对抗老年人和/或久坐不动者肌肉骨骼衰退的一种可行替代方法。应进一步开展研究，以加深对该领域的了解，并探索其他分子介质在振动生理适应性中的作用。
