Neilson Scarlet, Nangeroni Leah, Ghemrawi Mirna
Department of Chemistry & Physics, Arcadia University, 450 S Easton Rd, Glenside, PA 19038, USA.
The Center for Forensic Science Research and Education, 206 Welsh Road, Horsham, PA 19440, USA.
Methods Protoc. 2025 Jan 2;8(1):2. doi: 10.3390/mps8010002.
This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis. The efficiency of this procedure is demonstrated by the results within this paper, which highlights the successful recovery of both male autosomal and Y-STR profiles, even in mixed samples with a high female presence. Overall, this protocol demonstrates the effective recovery of DNA from sample pads, which is beneficial for forensic practitioners dealing with limited sample quantities, underscoring the value of using these pads in forensic analysis.
这种差异提取方案详细介绍了从侧向流动免疫色谱（LFI）测试中使用的样品垫中分离DNA的步骤，特别是对于涉及精液和月经血等混合生物样品或与性侵犯相关的其他证据的情况。该程序利用应用于免疫色谱测试样品垫的差异提取技术，其中样品垫作为底物。该方法包括两个连续的裂解步骤，以有效分离非精子和精子部分，从而能够针对不同细胞类型进行分离，以便进行下游DNA分析。本文的结果证明了该程序的效率，该结果突出显示即使在女性占比高的混合样品中，也成功恢复了男性常染色体和Y-STR图谱。总体而言，该方案证明了从样品垫中有效回收DNA，这对于处理有限样品量的法医从业者有益，强调了在法医分析中使用这些垫子的价值。
