Koczula Katarzyna M, Gallotta Andrea
Xeptagen SpA, VEGA Science Park, Via delle Industrie 9, Venice, Italy.
Xeptagen SpA, VEGA Science Park, Via delle Industrie 9, Venice, Italy
Essays Biochem. 2016 Jun 30;60(1):111-20. doi: 10.1042/EBC20150012.
Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of its potential to provide instantaneous diagnosis directly to patients. The range and interpretation of results and parameters used for evaluation of the assay will also be discussed. The main advantages and disadvantages of LFAs will be summarized and relevant future improvements to testing devices and strategies will be proposed. Finally, the major recent advances and future diagnostic applications in the LFA field will be explored.
侧向流动分析（LFA）是低成本、简单、快速且便携的检测设备背后的技术，这些设备在生物医学、农业、食品和环境科学领域颇受欢迎。本综述概述了该方法的原理以及分析的关键组件，重点是侧向流动免疫分析。由于这种分析方法有潜力直接为患者提供即时诊断，近来已引起了相当大的关注。还将讨论分析结果的范围和解读以及用于评估该分析的参数。将总结LFA的主要优缺点，并提出对测试设备和策略的相关未来改进建议。最后，将探讨LFA领域近期的主要进展和未来的诊断应用。
