Rodanaki Maria, Rask Eva, Lodefalk Maria
Department of Paediatrics, School of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
University Health Care Research Centre, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
J Pediatr Endocrinol Metab. 2025 Jan 24;38(3):288-291. doi: 10.1515/jpem-2024-0606. Print 2025 Mar 26.
Kisspeptin plays a major role in the onset of puberty by stimulating the gonadotropin-releasing hormone (GnRH) neurons. The aim of this study was to investigate whether GnRH inhibits kisspeptin secretion via a negative feedback mechanism and potential associations between kisspeptin levels and other hormones of importance for pubertal onset.
Thirteen girls with suspected central precocious puberty underwent a GnRH stimulation test twice in a randomized, placebo-controlled manner. Blood was sampled up to 150 min after an IV injection of either Relefact LHRH or saline. The levels of kisspeptin, acylated ghrelin, ultrasensitive oestradiol, luteinizing hormone (LH), follicle-stimulating hormone (FSH), insulin and glucose were analysed.
Baseline kisspeptin levels ranged from 9.9 to 201.6 pg/mL. Neither area under the curve for kisspeptin levels nor peaks were significantly lower after the GnRH injection compared to placebo. Baseline kisspeptin and glucose levels tended to be associated (rho=0.55, p=0.051) but no other associations were found between kisspeptin and other hormones.
Basal levels of kisspeptin vary widely in young girls. We found no evidence of a negative feedback mechanism of GnRH on kisspeptin in this small pilot study. The suggested association between kisspeptin and glucose levels needs further investigations.
kisspeptin通过刺激促性腺激素释放激素（GnRH）神经元在青春期启动过程中发挥主要作用。本研究的目的是调查GnRH是否通过负反馈机制抑制kisspeptin分泌，以及kisspeptin水平与青春期启动所涉及的其他重要激素之间的潜在关联。
13名疑似中枢性性早熟的女孩以随机、安慰剂对照的方式接受了两次GnRH刺激试验。静脉注射Relefact LHRH或生理盐水后150分钟内采集血液样本。分析了kisspeptin、酰化胃泌素、超敏雌二醇、促黄体生成素（LH）、促卵泡生成素（FSH）、胰岛素和葡萄糖的水平。
基线kisspeptin水平在9.9至201.6 pg/mL之间。与安慰剂相比，GnRH注射后kisspeptin水平的曲线下面积和峰值均无显著降低。基线kisspeptin水平与葡萄糖水平呈正相关趋势（rho=0.55，p=0.051），但未发现kisspeptin与其他激素之间存在其他关联。
年轻女孩中kisspeptin的基础水平差异很大。在这项小型初步研究中，我们没有发现GnRH对kisspeptin存在负反馈机制的证据。kisspeptin与葡萄糖水平之间的潜在关联需要进一步研究。
