Trajano Larissa Alexsandra da Silva Neto, Siqueira Priscyanne Barreto, Pinheiro Daphne, Farias Thayssa Gomes, Santos Márcia Soares Dos, Pires Bruno Ricardo Barreto, Fonseca Adenilson de Souza da, Mencalha Andre Luiz
Departamento de Biofísica e Biometria Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551030, Brazil.
Pró-Reitoria de Pesquisa e pós-graduação, Mestrado Profissional em Ciências Aplicadas em Saúde, Universidade de Vassouras, Avenida Expedicionário Oswaldo de Almeida Ramos, 280, Vassouras, Rio de Janeiro, 27700000, Brazil.
Lasers Med Sci. 2025 Jan 24;40(1):38. doi: 10.1007/s10103-025-04287-0.
In this article, we aim to evaluate the effects of photobiomodulation on mitochondria quantity, biogenesis, and mitophagy-associated genes in breast cancer (BC) cells. Both models were irradiated with a low-power infrared laser (880 nm, 150 mW) and amber LED (617 nm, 1500 mW), alone or simultaneously. We evaluated the mRNA expression of PINK1 and PGC-1α genes, and the mitochondrial number was assessed based on the ratio of mitochondrial DNA/genomic DNA (mtDNA/gDNA). No significant difference was observed in the mtDNA/gDNA ratio comparing the low-power infrared laser (LPIL) and LED-irradiated groups to their control counterparts. Similarly, no difference was observed in the mRNA levels of PINK1 and PGC-1α of the irradiated group with an LPIL and LED alone or in combination. In conclusion, PBM with LPIL and LED did not alter the mtDNA/gDNA ratio nor modulate the mRNA levels of the genes related to mitophagy and biogenesis in BC cells.
在本文中，我们旨在评估光生物调节对乳腺癌（BC）细胞中线粒体数量、生物发生及与线粒体自噬相关基因的影响。两种模型均用低功率红外激光（880纳米，150毫瓦）和琥珀色发光二极管（617纳米，1500毫瓦）单独或同时进行照射。我们评估了PINK1和PGC - 1α基因的mRNA表达，并根据线粒体DNA/基因组DNA的比率（mtDNA/gDNA）评估线粒体数量。与各自的对照相比，在低功率红外激光（LPIL）和发光二极管照射组的mtDNA/gDNA比率中未观察到显著差异。同样，单独或联合使用LPIL和发光二极管的照射组中，PINK1和PGC - 1α的mRNA水平也未观察到差异。总之，LPIL和发光二极管的光生物调节并未改变BC细胞中的mtDNA/gDNA比率，也未调节与线粒体自噬和生物发生相关基因的mRNA水平。
