Cao Thao Quyen, Eom Hyeongjin, Kim Hyukjin, Kang Ha Yeong, Park Young Min, Jung Sung Keun, Hahn Dongyup
Institute of Agricultural Science and Technology, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Integrative Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
J Microbiol Biotechnol. 2024 Dec 12;35:e2407062. doi: 10.4014/jmb.2407.07062.
L. (noni) is native to the tropical and semitropical areas and has been commercially available in health food stores and chain grocery stores specializing in natural foods, recently. Noni seeds are discarded as waste products through the industrial production of noni juice even though their bioactivity components might be a potential source of functional foods. Not many studies of phytochemistry and biological activity have been investigated on noni seeds until now. In this study, the phytochemical investigation of seeds led to the isolation of eight compounds (1-8) including four lignans (5-8). Their chemical structures were elucidated based on extensive spectroscopic analysis as well as the comparison with those reported in the literature. The isolated lignans were then evaluated for their anti-inflammatory activity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphynyltetrazolium bromide (MTT) assay in human bronchial epithelium BEAS-2B cells stimulated by 1-nitropyrene. As results, both four isolated lignans displayed high effects on the viability of BEAS-2B cells, indicating promising anti-inflammatory role in the airway disease.
诺丽原产于热带和亚热带地区，最近已在健康食品商店和专营天然食品的连锁杂货店中上市销售。尽管诺丽种子的生物活性成分可能是功能性食品的潜在来源，但在诺丽果汁的工业生产过程中，诺丽种子被作为废品丢弃。到目前为止，对诺丽种子的植物化学和生物活性的研究并不多。在本研究中，对诺丽种子进行植物化学研究，分离出了8种化合物（1-8），其中包括4种木脂素（5-8）。通过广泛的光谱分析以及与文献报道的结构进行比较，阐明了它们的化学结构。然后，通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法，在1-硝基芘刺激的人支气管上皮BEAS-2B细胞中，对分离得到的木脂素的抗炎活性进行了评估。结果显示，4种分离得到的木脂素对BEAS-2B细胞的活力均有显著影响，表明其在气道疾病中具有潜在的抗炎作用。
