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温室马铃薯蚜(Aulacorthum solani)的基因组序列草图

Draft genome sequence of the glasshouse-potato aphid Aulacorthum solani.

作者信息

Torres Joseph, Rozo-Lopez Paula, Brewer William, Saleh Ziabari Omid, Parker Benjamin J

机构信息

Department of Microbiology, University of Tennessee, Knoxville, Knoxville, TN 37916, USA.

Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA.

出版信息

G3 (Bethesda). 2025 Mar 18;15(3). doi: 10.1093/g3journal/jkaf013.

Abstract

Aulacorthum solani is a worldwide agricultural pest aphid capable of feeding on a wide range of host plants. This insect is a vector of plant viruses and causes injury to crops including stunted growth from the loss of phloem. We found that the publicly available genome for A. solani is contaminated with another aphid species, and we produced a new genome using a barcoded isogenic laboratory line. We generated Oxford Nanopore and Illumina reads to assemble a draft genome, and we sequenced RNA to aid in the annotation of our assembly. Our A. solani genome is 671 Mb containing 7,020 contigs with an N50 length of 196 kb with a BUSCO completeness of 98.6%. Out of the 24,981 genes predicted by EGAPx, 22,804 were annotated with putative functions based on homology to other aphid species. This genome will provide a useful resource for the community of researchers studying aphids from agricultural and genomic perspectives.

摘要

茄无网蚜是一种世界性的农业害虫蚜虫,能够取食多种寄主植物。这种昆虫是植物病毒的传播媒介,会对作物造成损害,包括因韧皮部损失导致生长发育迟缓。我们发现公开可用的茄无网蚜基因组被另一种蚜虫物种污染,于是我们使用带有条形码的同基因实验室品系构建了一个新的基因组。我们生成了牛津纳米孔测序和Illumina测序读数来组装基因组草图,并对RNA进行测序以辅助我们组装结果的注释。我们的茄无网蚜基因组大小为671 Mb,包含7020个重叠群,N50长度为196 kb,BUSCO完整性为98.6%。在EGAPx预测的24981个基因中,基于与其他蚜虫物种的同源性,有22804个基因被注释了推定功能。这个基因组将为从农业和基因组学角度研究蚜虫的研究人员群体提供有用的资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cda4/11917484/e6e53c66fc93/jkaf013f1.jpg

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