Tun Win, Vo Kieu Thi Xuan, Derakhshani Behnam, Yoon Jinmi, Cho Lae-Hyeon, Win Kay Tha Ye Soe, Lee Sang-Won, Jung Ki-Hong, Jeon Jong-Seong, An Gynheung
Graduate School of Green-Bio Science, Kyung Hee University, Yongin, Republic of Korea.
Department of Biological Sciences and Bioengineering, Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University, Incheon, Republic of Korea.
Front Plant Sci. 2025 Jan 9;15:1519039. doi: 10.3389/fpls.2024.1519039. eCollection 2024.
Plants are susceptible to infection by various pathogens with high epidemic potential. pv () causes bacterial blight in rice, one of the most significant diseases in both temperate and tropical regions. In this study, we report the identification and characterization of , a sucrose-inducible transcription factor, that plays a role in the plant defense responses following infection. We found that mutant plants with defective showed enhanced defense response specifically to , indicating that this transcription factor acts as a negative defense regulator. In contrast, mutant plants did not exhibit higher resistance compared to wild-type (WT) plants when infected with the rice blast fungal pathogen . Transcriptomic analysis of mutant and WT plants revealed that several pathogen resistance genes were upregulated in mutants. Of these, we selected for further analysis. Transient expression experiments in rice protoplasts showed that OsWRKY26 repressed the expression of a reporter gene driven by the promoter. Chromatin immunoprecipitation analysis revealed that OsWRKY26 binds directly to the promoter region of . These findings suggest that OsWRKY26 negatively regulates the defense response during infection by repressing as well as other pathogen-related genes such as , , , , , , and
植物易受具有高流行潜力的各种病原体感染。稻黄单胞菌（Xanthomonas oryzae pv.）引起水稻白叶枯病，这是温带和热带地区最重要的病害之一。在本研究中，我们报告了蔗糖诱导型转录因子OsWRKY26的鉴定和特征，其在稻黄单胞菌感染后的植物防御反应中发挥作用。我们发现，OsWRKY26功能缺陷的突变植物对稻黄单胞菌表现出增强的防御反应，表明该转录因子作为负防御调节因子发挥作用。相比之下，当用稻瘟病菌感染时，突变植物与野生型（WT）植物相比没有表现出更高的抗性。对突变体和野生型植物的转录组分析表明，几个病原体抗性基因在突变体中上调。其中，我们选择OsWRKY26进行进一步分析。水稻原生质体中的瞬时表达实验表明，OsWRKY26抑制由OsWRKY26启动子驱动的报告基因的表达。染色质免疫沉淀分析表明，OsWRKY26直接结合OsWRKY26的启动子区域。这些发现表明，OsWRKY26通过抑制OsWRKY26以及其他病原体相关基因如OsWRKY45、OsWRKY62、OsWRKY72、PR1a、PR1b、PR3和PR10，在稻黄单胞菌感染期间负调控防御反应。
