Fem细胞表面信号系统在[具体环境]中受ExsA调控，并影响致病性。（注：原文中“in”后面缺少具体内容）

The Fem cell-surface signaling system is regulated by ExsA in and affects pathogenicity.

作者信息

Li Yanqi, Bhagirath Anjali, Badr Sara, Zhang Pansong, Chen Lin, Dadashi Maryam, Surette Michael G, Duan Kangmin

机构信息

Department of Oral Biology, Dr. Gerald Niznick College of Dentistry, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0W2, Canada.

Dalhousie University, Faculty of Dentistry, Halifax, NS B3H 1W2, Canada.

出版信息

iScience. 2024 Dec 18;28(1):111629. doi: 10.1016/j.isci.2024.111629. eCollection 2025 Jan 17.

DOI:10.1016/j.isci.2024.111629

PMID:39850353

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11754118/

Abstract

Bacterial interspecies interactions shape microbial communities and influence the progression of polymicrobial infections. FemI-FemR-FemA, a cell-surface signaling system, in , is involved in the uptake of iron-chelating mycobactin produced by spp. In this report, we present the data that indicates the -PA1909 operon is positively regulated by ExsA, a master regulator for the type three secretion system (T3SS), connecting the Fem system with T3SS. Intriguingly, the Fem system also influenced virulence factors in including the quorum sensing systems, pyocyanin production, biofilm formation, and the type six secretion systems (T6SSs). Using a infection model we observed that a deletion in PAO1 significantly increased the host survival rate while over-expression decreased it, suggesting a role for the Fem system in pathogenicity . Our data indicate the Fem system is a target of the T3SS master activator ExsA, and it affects pathogenicity.

摘要

细菌种间相互作用塑造微生物群落并影响多微生物感染的进程。FemI-FemR-FemA是一种细胞表面信号系统，参与摄取由分枝杆菌属产生的铁螯合分枝杆菌素。在本报告中，我们展示的数据表明，-PA1909操纵子受三型分泌系统（T3SS）的主要调节因子ExsA正向调节，将Fem系统与T3SS联系起来。有趣的是，Fem系统还影响铜绿假单胞菌中的毒力因子，包括群体感应系统、绿脓菌素产生、生物膜形成和六型分泌系统（T6SS）。使用铜绿假单胞菌感染模型，我们观察到PAO1中的一个缺失显著提高了宿主存活率，而其过表达则降低了宿主存活率，这表明Fem系统在致病性中起作用。我们的数据表明，Fem系统是T3SS主要激活因子ExsA的靶点，并且它影响铜绿假单胞菌的致病性。