Akaike Mami, Hatakeyama Jun, Saito Yoichi, Nakanishi Yoshitaka, Shimamura Kenji, Nakashima Yuta
Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Chuo-ku, Kumamoto 860-8555, Japan.
Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjyo, Chuo-ku, Kumamoto 860-0811, Japan.
Bioengineering (Basel). 2024 Dec 24;12(1):3. doi: 10.3390/bioengineering12010003.
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The device comprises a glass substrate and a microchannel constructed of polydimethylsiloxane, polytetrafluoroethylene tubes, a glass capillary, and a microsyringe pump. This device obviates the need for electrical measurements, relying solely on the displacement of ultrapure water within the microchannel to assess the micropressure in embryos. First, the device was subjected to arbitrary pressures, and the relationship between the pressure and the displacement of ultrapure water in the microchannel was determined. Calibration results showed that the displacement [μm] could be calculated from the pressure [Pa] using the equation = 0.36 . The coefficient of determination was shown to be 0.87, indicating a linear response. When utilized to measure brain ventricular pressure in mouse embryos, the fabricated device yielded an average pressure reading of 1313 ± 640 Pa. This device can facilitate the measurement of pressure within microcavities in living tissues and other areas requiring precise and localized pressure evaluations.
机械力影响体内细胞的增殖、分化、组织形态发生和功能表达。为了理解这些力对生物体的影响，对其进行量化至关重要。本研究介绍了一种专为细胞尺度压力评估量身定制的新型微差压测量装置。该装置包括一个玻璃基板和一个由聚二甲基硅氧烷、聚四氟乙烯管、玻璃毛细管和微注射器泵构成的微通道。该装置无需进行电测量，仅依靠微通道内超纯水的位移来评估胚胎中的微压力。首先，对该装置施加任意压力，并确定压力与微通道内超纯水位移之间的关系。校准结果表明，位移[μm]可使用方程 = 0.36从压力[Pa]计算得出。测定系数显示为0.87，表明呈线性响应。当用于测量小鼠胚胎的脑室压力时，所制造的装置产生的平均压力读数为1313±640 Pa。该装置可便于测量活组织微腔内以及其他需要精确和局部压力评估的区域内的压力。
