通过可视化技术和工具探索RNA测序数据分析:临床应用的机遇与局限的系统综述
Exploring RNA-Seq Data Analysis Through Visualization Techniques and Tools: A Systematic Review of Opportunities and Limitations for Clinical Applications.
作者信息
Manzoor Farhana, Tsurgeon Cyruss A, Gupta Vibhuti
机构信息
Department of Computer Science and Data Science, School of Applied Computational Sciences, Meharry Medical College, Nashville, TN 37208, USA.
Department of Biomedical Data Science, School of Applied Computational Sciences, Meharry Medical College, Nashville, TN 37208, USA.
出版信息
Bioengineering (Basel). 2025 Jan 12;12(1):56. doi: 10.3390/bioengineering12010056.
RNA sequencing (RNA-seq) has emerged as a prominent resource for transcriptomic analysis due to its ability to measure gene expression in a highly sensitive and accurate manner. With the increasing availability of RNA-seq data analysis from clinical studies and patient samples, the development of effective visualization tools for RNA-seq analysis has become increasingly important to help clinicians and biomedical researchers better understand the complex patterns of gene expression associated with health and disease. This review aims to outline the current state-of-the-art data visualization techniques and tools commonly used to frame clinical inferences from RNA-seq data and point out their benefits, applications, and limitations. A systematic review of English articles using PubMed, Scopus, Web of Science, and IEEE Xplore databases was performed. Search terms included "RNA-seq", "visualization", "plots", and "clinical". Only full-text studies reported between 2017 and 2024 were included for analysis. Following PRISMA guidelines, a total of 126 studies were identified, of which 33 studies met the inclusion criteria. We found that 18% of studies have visualization techniques and tools for circular RNA-seq data, 56% for single-cell RNA-seq data, 23% for bulk RNA-seq data, and 3% for long non-coding RNA-seq data. Overall, this review provides a comprehensive overview of the common visualization tools and their potential applications, which is a useful resource for researchers and clinicians interested in using RNA-seq data for various clinical purposes (e.g., diagnosis or prognosis).
RNA测序(RNA-seq)因其能够以高度灵敏和准确的方式测量基因表达,已成为转录组分析的重要资源。随着临床研究和患者样本中RNA-seq数据分析的可用性不断提高,开发有效的RNA-seq分析可视化工具对于帮助临床医生和生物医学研究人员更好地理解与健康和疾病相关的复杂基因表达模式变得越来越重要。本综述旨在概述当前用于从RNA-seq数据构建临床推断的最先进数据可视化技术和工具,并指出它们的优点、应用和局限性。我们使用PubMed、Scopus、Web of Science和IEEE Xplore数据库对英文文章进行了系统综述。搜索词包括“RNA-seq”、“可视化”、“图表”和“临床”。仅纳入2017年至2024年间报道的全文研究进行分析。按照PRISMA指南,共确定了126项研究,其中33项研究符合纳入标准。我们发现,18%的研究有用于环状RNA-seq数据的可视化技术和工具,56%用于单细胞RNA-seq数据,23%用于大量RNA-seq数据,3%用于长链非编码RNA-seq数据。总体而言,本综述全面概述了常见的可视化工具及其潜在应用,对于有兴趣将RNA-seq数据用于各种临床目的(如诊断或预后)的研究人员和临床医生来说是一个有用的资源。