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氯化钙交联溶液浓度对包埋于海藻酸盐基水凝胶中的16HBE14o-人支气管细胞长期细胞活力的影响

Effects of Calcium Chloride Crosslinking Solution Concentration on the Long-Term Cell Viability of 16HBE14o- Human Bronchial Cells Embedded in Alginate-Based Hydrogels.

作者信息

Wood Nathan, Doria Esther I, Rahman Taieba Tuba, Li Wanhe, Pei Zhijian, Qin Hongmin

机构信息

Department of Biology, Texas A&M University, 3258 TAMU, College Station, TX 77843, USA.

Department of Industrial & Systems Engineering, Texas A&M University, 3127 TAMU, College Station, TX 77843, USA.

出版信息

Biomimetics (Basel). 2025 Jan 10;10(1):40. doi: 10.3390/biomimetics10010040.

DOI:10.3390/biomimetics10010040
PMID:39851756
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11763177/
Abstract

In this preliminary study, the long-term effects of calcium chloride crosslinking concentration on viability of 16HBE14o- human bronchial epithelial cells embedded in alginate-extracellular matrix (ECM) or alginate-methylcellulose-ECM hydrogels have been investigated. There is currently a limited understanding regarding the effects of crosslinking solution concentration on lung epithelial cells embedded in hydrogel. Furthermore, the effects of calcium chloride concentration in crosslinking solutions on other cell types have not been reported regarding whether the addition of viscosity and stiffness tuning agents such as methylcellulose will alter the responses of cells to changes in calcium chloride concentration in crosslinking solutions. While there were no significant effects of calcium chloride concentration on cell viability in alginate-ECM hydrogels, there is a decrease in cell viability in alginate-methylcellulose-ECM hydrogels crosslinked with 300 mM calcium chloride crosslinking solution. These findings have implications in the maintenance of 16HBE14o- 3D cultures with respect to the gelation of alginate with high concentrations of ionic crosslinking solution.

摘要

在这项初步研究中，研究了氯化钙交联浓度对包埋于海藻酸盐-细胞外基质（ECM）或海藻酸盐-甲基纤维素-ECM水凝胶中的16HBE14o-人支气管上皮细胞活力的长期影响。目前，对于交联溶液浓度对包埋于水凝胶中的肺上皮细胞的影响了解有限。此外，关于交联溶液中氯化钙浓度对其他细胞类型的影响，尚未有研究报道添加诸如甲基纤维素等粘度和硬度调节剂是否会改变细胞对交联溶液中氯化钙浓度变化的反应。虽然氯化钙浓度对海藻酸盐-ECM水凝胶中的细胞活力没有显著影响，但用300 mM氯化钙交联溶液交联的海藻酸盐-甲基纤维素-ECM水凝胶中的细胞活力有所下降。这些发现对于使用高浓度离子交联溶液使海藻酸盐凝胶化来维持16HBE14o-三维培养具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/44f111d176af/biomimetics-10-00040-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/9e4e71d55af1/biomimetics-10-00040-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/2bd7991107e9/biomimetics-10-00040-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/aee2b2849186/biomimetics-10-00040-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/7b1cf41381a5/biomimetics-10-00040-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/7dd7c2863250/biomimetics-10-00040-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/cd82c6d035a9/biomimetics-10-00040-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/d76ae273537d/biomimetics-10-00040-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/62cc21e409ca/biomimetics-10-00040-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/44f111d176af/biomimetics-10-00040-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/9e4e71d55af1/biomimetics-10-00040-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/2bd7991107e9/biomimetics-10-00040-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/aee2b2849186/biomimetics-10-00040-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/7b1cf41381a5/biomimetics-10-00040-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/7dd7c2863250/biomimetics-10-00040-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/cd82c6d035a9/biomimetics-10-00040-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/d76ae273537d/biomimetics-10-00040-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/62cc21e409ca/biomimetics-10-00040-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b5c2/11763177/44f111d176af/biomimetics-10-00040-g009.jpg

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