Natural Polysaccharide-Based Hydrogels for Accelerating Hemostasis.

Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.5%, 3.0%, and 4.0%). A rotational rheometer was used to describe the hydrogels' shape and rheological characteristics. At all concentrations, it was found that the hydrogels' elastic modulus (G') consistently and significantly outperformed their viscous modulus (G″), suggesting a robust internal structure. All of the hydrogels had cell viability levels as high as 100% and hemolysis rates below 1%, indicating the hydrogels' outstanding biocompatibility. Furthermore, the hydrogels demonstrated superior hemostatic qualities in an in vivo mouse tail amputation model, as well as in in vitro coagulation tests. The results show that the hydrogel possesses excellent self-healing properties, as well as a good biocompatibility and hemostatic performance, thus paving the way for the development of a potential hemostatic green hydrogel.