Lin Hui-Fang, Wang Yue-Yue, Liu Feng-Zhen, Yang Zi-Wei, Cui Hao, Hu Si-Yu, Li Feng-He, Pan Pei
School of Pharmacy, Anhui Medical University, Hefei 230032, China.
Gels. 2025 Jan 8;11(1):48. doi: 10.3390/gels11010048.
Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.5%, 3.0%, and 4.0%). A rotational rheometer was used to describe the hydrogels' shape and rheological characteristics. At all concentrations, it was found that the hydrogels' elastic modulus (G') consistently and significantly outperformed their viscous modulus (G″), suggesting a robust internal structure. All of the hydrogels had cell viability levels as high as 100% and hemolysis rates below 1%, indicating the hydrogels' outstanding biocompatibility. Furthermore, the hydrogels demonstrated superior hemostatic qualities in an in vivo mouse tail amputation model, as well as in in vitro coagulation tests. The results show that the hydrogel possesses excellent self-healing properties, as well as a good biocompatibility and hemostatic performance, thus paving the way for the development of a potential hemostatic green hydrogel.
大多数现有的水凝胶敷料在机械性能、生物活性、兼容性或多功能性方面存在不足，这使得开发用于生化和生物医学应用的快速、绿色且经济高效的水凝胶方法成为当务之急。在此，受天然多糖水凝胶固有的生物活性、保水性能和生物相容性的启发，我们制备了自愈合凝胶。通过多糖（BSP）、羧甲基壳聚糖（CMCS）和硼砂经由硼酸酯键，我们制备了不同浓度（2.5%、3.0%和4.0%）的具有止血和自愈合功能的中药水凝胶。使用旋转流变仪来描述水凝胶的形态和流变特性。在所有浓度下，发现水凝胶的弹性模量（G'）始终显著优于其粘性模量（G''），表明其内部结构坚固。所有水凝胶的细胞活力水平高达100%，溶血率低于1%，表明水凝胶具有出色的生物相容性。此外，水凝胶在体内小鼠尾巴截肢模型以及体外凝血试验中均表现出优异的止血性能。结果表明，该水凝胶具有优异的自愈合性能、良好的生物相容性和止血性能，从而为开发潜在的止血绿色水凝胶铺平了道路。
