Wang Yajun, Liu Jingjing, Wang Zhendong, Zhang Mei, Zhang Yongzhuo
College of Information and Control Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
College of Information and Control Engineering, China Northeast Electric Power University, Jilin 132011, China.
Biosensors (Basel). 2024 Dec 25;15(1):4. doi: 10.3390/bios15010004.
Single-molecule sequencing technology, a novel method for gene sequencing, utilizes nano-sized materials to detect electrical and fluorescent signals. Compared to traditional Sanger sequencing and next-generation sequencing technologies, it offers significant advantages, including ultra-long read lengths, rapid sequencing, and the absence of amplification steps, making it widely applicable across various fields. By examining the development and components of single-molecule sequencing technology, it becomes clear that its unique characteristics provide new opportunities for advancing metrological traceability. Notably, its direct detection capabilities offer a novel approach to nucleic acid metrology. This paper provides a detailed overview of library construction, signal generation and detection, and data analysis methods in single-molecule sequencing and discusses its implications for nucleic acid metrology.
单分子测序技术是一种新型基因测序方法，它利用纳米材料来检测电信号和荧光信号。与传统的桑格测序法和新一代测序技术相比，它具有显著优势，包括超长读长、快速测序以及无需扩增步骤，因而在各个领域都有广泛应用。通过研究单分子测序技术的发展历程和组成部分可以发现，其独特特性为推进计量溯源提供了新机遇。值得注意的是，其直接检测能力为核酸计量提供了一种新方法。本文详细概述了单分子测序中的文库构建、信号生成与检测以及数据分析方法，并探讨了其对核酸计量的意义。
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