Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom.
Department of Materials, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom.
Nano Lett. 2023 Jul 26;23(14):6760-6767. doi: 10.1021/acs.nanolett.3c01271. Epub 2023 Jun 6.
Easily deploying new vaccines globally to combat disease outbreaks has been highlighted as a major necessity by the World Health Organization. RNA-based vaccines using lipid nanoparticles (LNPs) as a drug delivery system were employed to great effect during the recent COVID-19 pandemic. However, LNPs are still unstable at room temperature and agglomerate over time during storage, rendering them ineffective for intracellular delivery. We demonstrate the suitability of nanohole arrays (nanopackaging) as patterned surfaces to separate and store functionalized LNPs (fLNPs) in individual recesses, which can be expanded to other therapeutics. Encapsulating calcein as a model drug, we show through confocal microscopy the effective loading of fLNPs into our nanopackaging for both wet and dry systems. We prove quantifiably pH-mediated capture and subsequent unloading of over 30% of the fLNPs using QCM-D on alumina surfaces altering the pH from 5.5 to 7, displaying controllable storage at the nanoscale.
世界卫生组织强调，要在全球范围内轻松部署新疫苗以应对疾病爆发，这是一项主要需求。在最近的 COVID-19 大流行期间，使用脂质纳米颗粒 (LNP) 作为药物递送系统的基于 RNA 的疫苗被大量使用。然而，LNP 在室温下仍然不稳定，并且在储存过程中会随时间聚集，从而使其无法有效进行细胞内递送。我们证明了纳米孔阵列（纳米封装）作为分离和储存功能化 LNP（fLNP）的图案化表面的适用性，这些纳米孔可以扩展到其他治疗方法。我们将 calcein 包封作为模型药物，通过共聚焦显微镜显示了 fLNP 有效装载到我们的纳米封装中的情况，无论是在湿系统还是干系统中。我们通过 QCM-D 在氧化铝表面上证明了可定量 pH 介导的捕获和随后卸载，从 5.5 到 7 改变 pH，在纳米尺度上显示出可控的存储。
