Pushparajah Suthira, Shafiei Mahnaz, Yu Aimin
School of Science, Computing, and Engineering Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia.
Biosensors (Basel). 2025 Jan 3;15(1):15. doi: 10.3390/bios15010015.
Carbendazim (CBZ) is used to prevent fungal infections in agricultural crops. Given its high persistence and potential for long-term health effects, it is crucial to quickly identify pesticide residues in food and the environment in order to mitigate excessive exposure. Aptamer-based sensors offer a promising solution for pesticide detection due to their exceptional selectivity, design versatility, ease of use, and affordability. Herein, we report the development of an electrochemical aptasensor for CBZ detection. The sensor was fabricated through a one-step electrodeposition of platinum nanoparticles (Pt NPs) and reduced graphene oxide (rGO) on a glassy carbon electrode (GCE). Then, a CBZ-specific aptamer was attached via Pt-sulfur bonds. Upon combining CBZ with the aptamer on the electrode surface, the redox reaction of the electrochemical probe K[Fe(CN)] is hindered, resulting in a current drop. Under optimized conditions (pH of 7.5 and 25 min of incubation time), the proposed aptasensor showed a linear current reduction to CBZ concentrations between 0.5 and 15 nM. The limit of detection (LOD) for this proposed aptasensor is 0.41 nM. Along with its repeatable character, the aptasensor demonstrated better selectivity for CBZ compared to other potential compounds. The recovery rates for detecting CBZ in skim milk and tap water using the standard addition method were 98% and 96%, respectively. The proposed aptasensor demonstrated simplicity, sensitivity, and selectivity for detecting CBZ with satisfactory repeatability. It establishes a strong foundation for environmental monitoring of CBZ.
多菌灵（CBZ）用于预防农作物中的真菌感染。鉴于其高持久性和对健康的长期潜在影响，快速识别食品和环境中的农药残留以减少过度暴露至关重要。基于适配体的传感器因其卓越的选择性、设计的多功能性、易用性和经济性，为农药检测提供了一个有前景的解决方案。在此，我们报告了一种用于检测CBZ的电化学适配体传感器的开发。该传感器通过在玻碳电极（GCE）上一步电沉积铂纳米颗粒（Pt NPs）和还原氧化石墨烯（rGO）制成。然后，通过Pt - 硫键连接CBZ特异性适配体。当CBZ与电极表面的适配体结合时，电化学探针K[Fe(CN)]的氧化还原反应受到阻碍，导致电流下降。在优化条件下（pH为7.5，孵育时间为25分钟），所提出的适配体传感器对CBZ浓度在0.5至15 nM之间呈现出线性电流降低。该适配体传感器的检测限（LOD）为0.41 nM。除了具有可重复的特性外，与其他潜在化合物相比，该适配体传感器对CBZ表现出更好的选择性。使用标准加入法在脱脂牛奶和自来水中检测CBZ的回收率分别为98%和96%。所提出的适配体传感器在检测CBZ时表现出简单性、灵敏度和选择性，具有令人满意的可重复性。它为CBZ的环境监测奠定了坚实的基础。
