Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, China.
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Science, Jiangxi Agricultural University, Nanchang 330045, China.
Molecules. 2022 Oct 12;27(20):6842. doi: 10.3390/molecules27206842.
A facile and sensitive electrochemical aptamer sensor (aptasensor) based on Au nanoparticles-decorated porous carbon (AuNPs/PC) composite was developed for the efficient determination of the antibiotic drug chloramphenicol (CAP). AuNPs modified metal-organic framework (AuNPs/ZIF-8) is applied as a precursor to synthesize the porous carbon with homogeneous AuNPs distribution through a direct carbonization step under nitrogen atmosphere. The as-synthesized AuNPs/PC exhibits high surface area and improved conductivity. Moreover, the loading AuNPs could enhance the attachment of the aptamers on the surface of electrode through the Au-S bond. When added to CAP, poorly conductive aptamer-CAP complexes are formed on the sensor surface, which increases the hindrance to electron transfer resulting in a decrease in electrochemical signal. Based on this mechanism, the developed CAP aptasensor represents a wide linear detection range of 0.1 pM to 100 nM with a low detection limit of 0.03 pM (S/N = 3). In addition, the proposed aptasensor was employed for the analysis of CAP in honey samples and provided satisfactory recovery.
一种基于金纳米粒子修饰的多孔碳（AuNPs/PC）复合材料的简便灵敏电化学适体传感器（aptasensor）被开发出来，用于高效测定抗生素药物氯霉素（CAP）。AuNPs 修饰的金属有机骨架（AuNPs/ZIF-8）被用作前驱体，通过在氮气气氛下的直接碳化步骤合成具有均匀 AuNPs 分布的多孔碳。所合成的 AuNPs/PC 具有高比表面积和改善的导电性。此外，负载的 AuNPs 可以通过 Au-S 键增强适体在电极表面的附着。当添加到 CAP 时，在传感器表面形成了不良导电的适体-CAP 配合物，这增加了电子转移的阻碍，导致电化学信号降低。基于这一机制，所开发的 CAP aptasensor 具有从 0.1 pM 到 100 nM 的宽线性检测范围，检测限低至 0.03 pM（S/N = 3）。此外，该适体传感器还用于蜂蜜样品中 CAP 的分析，提供了令人满意的回收率。
