Zhang Yuyao, Qian Li, Zhang Qian, Li Yu, Liu Yu, Jiang Dechen
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210092, China.
Sir Run Run Hospital, Nanjing Medical University, Nanjing 211100, China.
Biosensors (Basel). 2025 Jan 15;15(1):47. doi: 10.3390/bios15010047.
Herein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initial ECL reaction. The electrochemical characterizations confirm the loose structure of the assembled layer with the immune complex, providing an increase in the current and the resultant enhanced ECL emissions. Comparing the sensors with the rigid structure, a 34-fold increase in the maximal ECL emission is recorded when PEG3400 is used as a linker. Using the optimized protocol, the prepared immunosensor exhibits a wide-ranging linear response to the model antibody (glutamate decarboxylase antibody) ranging from 10 pg/mL to 10 ng/mL. The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance.
在此，通过将钌标记的免疫复合物固定在电极表面的柔性聚乙二醇（PEG）链上，设计了一种灵敏的电化学发光（ECL）免疫传感器，这为初始ECL反应期间钌配合物在电极上的碰撞提供了更大的自由度。电化学表征证实了组装层与免疫复合物的松散结构，导致电流增加以及由此增强的ECL发射。与具有刚性结构的传感器相比，当使用PEG3400作为连接子时，最大ECL发射记录增加了34倍。使用优化方案，制备的免疫传感器对模型抗体（谷氨酸脱羧酶抗体）表现出从10 pg/mL到10 ng/mL的广泛线性响应。检测限比使用经典酶联免疫吸附测定的值低近两个数量级，这为增强ECL发射和由此产生的分析性能提供了一种新设计。
Zotero 插件