Gou Xiaodan, Xing Zejing, Ma Cheng, Zhu Jun-Jie
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P.R. China.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, P.R. China.
Chem Biomed Imaging. 2023 Mar 6;1(5):414-433. doi: 10.1021/cbmi.2c00007. eCollection 2023 Aug 28.
Electrochemiluminescence (ECL) is a typical luminescence process triggered by electrochemical reactions. Due to the separated signal types, ECL measurements have some merits of high sensitivity, low background, and simple configuration. Coupled with a microscopy setup, ECL microscopy (ECLM) has the unique characteristics of ECL and is also furnished with spatiotemporal resolution. Thus, many applications have been created, including nanoscale sensing, ECL mechanism deciphering, transient events of single objects, and ECLM crossover methods. In this review, we will overview the development and basic knowledge of ECL and then profile the setup design of ECLM. Through the understanding of these two parts, we will next probe the diverse applications of ECLM, combining the inter-relation with each other. Finally, the outlook discussing the expectations of further progress of ECLM technology.
电化学发光（ECL）是一种由电化学反应引发的典型发光过程。由于信号类型相互分离，ECL测量具有高灵敏度、低背景和配置简单等优点。与显微镜装置相结合，ECL显微镜（ECLM）具有ECL的独特特性，还具备时空分辨率。因此，已经产生了许多应用，包括纳米级传感、ECL机制解析、单个物体的瞬态事件以及ECLM交叉方法。在本综述中，我们将概述ECL的发展和基础知识，然后介绍ECLM的装置设计。通过对这两部分的理解，我们接下来将探讨ECLM的各种应用，并结合它们之间的相互关系。最后，展望部分将讨论对ECLM技术进一步发展的期望。
