Coleman Laura Jane, Byrne John L, Edwards Stuart, O'Hara Rosemary
Department of Applied Science, South East Technological University, R93 V960 Carlow, Ireland.
UPMC Aut Even Hospital, R95 D370 Kilkenny, Ireland.
J Pers Med. 2025 Jan 4;15(1):17. doi: 10.3390/jpm15010017.
This study investigated the inflammatory responses of fibroblast-like synoviocytes (FLS) isolated from osteoarthritis (OA) patients, stimulated with lipopolysaccharide (LPS) and interleukin-6 (IL-6). Both experimental and synthetic data were utilised to investigate the variability in IL-6 and myeloperoxidase (MPO) production and its implications for OA pathogenesis. Synovial biopsies were obtained from OA patients undergoing joint replacement surgery. FLS were isolated, cultured, and stimulated with varying concentrations of LPS and IL-6. The production of IL-6 and MPO was measured using enzyme-linked immunosorbent assays (ELISA). Synthetic data generation techniques expanded the dataset to support comprehensive statistical analyses. The patterns of inflammatory responses revealed distinct patient subgroups, highlighting individual variability. The integration of synthetic data with experimental observations validated their reliability and demonstrated dose-dependent differences in IL-6 and MPO production across patients. The results highlighted the importance of patient-specific factors in OA inflammation and demonstrated the utility of combining experimental and synthetic data to model individual variability. The results support the development of personalised treatment strategies in OA. Future research should include larger patient datasets and an exploration of molecular mechanisms underlying these responses.
本研究调查了从骨关节炎（OA）患者分离出的成纤维样滑膜细胞（FLS）在脂多糖（LPS）和白细胞介素-6（IL-6）刺激下的炎症反应。利用实验数据和合成数据来研究IL-6和髓过氧化物酶（MPO）产生的变异性及其对OA发病机制的影响。从接受关节置换手术的OA患者获取滑膜活检组织。分离、培养FLS，并用不同浓度的LPS和IL-6进行刺激。使用酶联免疫吸附测定（ELISA）测量IL-6和MPO的产生。合成数据生成技术扩展了数据集，以支持全面的统计分析。炎症反应模式揭示了不同的患者亚组，突出了个体变异性。将合成数据与实验观察结果相结合，验证了其可靠性，并证明了患者间IL-6和MPO产生存在剂量依赖性差异。结果突出了患者特异性因素在OA炎症中的重要性，并证明了结合实验数据和合成数据来模拟个体变异性的实用性。这些结果支持OA个性化治疗策略的制定。未来的研究应纳入更大的患者数据集，并探索这些反应背后的分子机制。
