Esquivel-Naranjo Edgardo Ulises, Mancilla-Diaz Hector, Marquez-Mazlin Rudi, Alizadeh Hossein, Kandula Diwakar, Hampton John, Mendoza-Mendoza Artemio
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
Unit for Basic and Applied Microbiology, Faculty of Natural Sciences, Autonomous University of Queretaro, Queretaro 76230, Mexico.
J Fungi (Basel). 2024 Dec 26;11(1):9. doi: 10.3390/jof11010009.
Secondary metabolism is one of the main mechanisms uses to explore and colonize new niches, and 6-pentyl-α-pyrone (6-PP) is an important secondary metabolite in this process. This work focused on standardizing a method to investigate the production of 6-PP. Ethanol and ethyl acetate were both effective solvents for quantifying 6-PP in solution and had limited solubility in potato-dextrose-broth media. The 6-PP extraction using ethyl acetate provided a rapid and efficient process to recover this metabolite. The 6-PP was readily produced during the development of growing in the dark, but light suppressed its production. The 6-PP was purified, and its spectrum by nuclear magnetic resonance and mass spectroscopy was identical to that of commercial 6-PP. Light also induced or suppressed other unidentified metabolites in several other species of . The antagonistic activity of was influenced by light, as suppression of plant pathogens was greater in the dark. The secreted metabolite production on potato-dextrose-agar was differentially regulated by light, indicating that produced several metabolites with antagonistic activity against plant pathogens. Light has an important influence on the secondary metabolism and antagonistic activity of , and this trait is of key relevance for selecting antagonistic strains for plant protection.
次生代谢是用于探索和定殖新生态位的主要机制之一，6-戊基-α-吡喃酮（6-PP）是这一过程中的一种重要次生代谢产物。这项工作着重于规范一种研究6-PP产生的方法。乙醇和乙酸乙酯都是定量溶液中6-PP的有效溶剂，且在马铃薯葡萄糖肉汤培养基中的溶解度有限。用乙酸乙酯提取6-PP提供了一种快速有效的回收该代谢产物的方法。在黑暗中生长发育期间很容易产生6-PP，但光照会抑制其产生。6-PP被纯化，其核磁共振和质谱光谱与市售6-PP的光谱相同。光照还会诱导或抑制其他几种未鉴定的代谢产物。的拮抗活性受光照影响，因为在黑暗中对植物病原体的抑制作用更大。马铃薯葡萄糖琼脂上分泌的代谢产物产生受光照差异调节，表明产生了几种对植物病原体具有拮抗活性的代谢产物。光照对的次生代谢和拮抗活性有重要影响，这一特性对于选择用于植物保护的拮抗菌株至关重要。
