Porter C H, Collins R C
Am J Trop Med Hyg. 1985 Mar;34(2):302-9. doi: 10.4269/ajtmh.1985.34.302.
A total of 1,700 host-seeking Simulium ochraceum were allowed to feed to repletion on human volunteers heavily infected with Onchocerca volvulus and then were marked and released. Seventy-three (4.3%) of the flies were recaptured. The gonotrophic cycle of S. ochraceum, including the host seeking and oviposition phases, was approximately 74-97 hr in duration; however, for a few flies the cycle was up to an additional 24 hr in length. The frequency distribution of recaptured flies indicated that 2 gonotrophic cycles may be completed in 7-8 days. The large proportion of flies recaptured with sac-stage follicular dilatations indicates that S. ochraceum may begin to search for and can rapidly locate a new host almost immediately after oviposition. Larvae of O. volvulus were found in 15 (20.6%) of the recaptured flies. Early first stage larvae were present in host-seeking flies that had completed 1 gonotrophic cycle: 12 of 13 infected flies recaptured between 74 and 97 hr had early first stage larvae. Development of O. volvulus larvae to the infective stage was estimated to require approximately 11 days at the study site. During this time, S. ochraceum would normally have completed 3 gonotrophic cycles and be in the host seeking phase of a fourth at approximately 11 days after ingesting microfilariae.
让总共1700只寻找宿主的金黄蚋吸食大量感染盘尾丝虫的人类志愿者的血液直至饱腹，然后对其进行标记并释放。其中73只（4.3%）苍蝇被重新捕获。金黄蚋的生殖营养周期，包括寻找宿主和产卵阶段，持续时间约为74 - 97小时；然而，少数苍蝇的周期长度可额外延长24小时。重新捕获苍蝇的频率分布表明，在7 - 8天内可能完成2个生殖营养周期。大量重新捕获的苍蝇出现囊期卵泡扩张，这表明金黄蚋在产卵后可能几乎立即开始寻找并能迅速找到新宿主。在15只（20.6%）重新捕获的苍蝇中发现了盘尾丝虫幼虫。在完成1个生殖营养周期的寻找宿主的苍蝇中存在早期第一阶段幼虫：在74至97小时之间重新捕获的13只受感染苍蝇中有12只带有早期第一阶段幼虫。在研究地点，盘尾丝虫幼虫发育到感染阶段估计需要约11天。在此期间，金黄蚋通常会完成3个生殖营养周期，并在摄入微丝蚴后约11天进入第四个生殖营养周期的寻找宿主阶段。
