Sadek P C, Carr P W, Bowers L D, Haddad L C
Anal Biochem. 1985 Jan;144(1):128-31. doi: 10.1016/0003-2697(85)90093-4.
Much success has been achieved in the separation and purification of a wide range of proteins using various high-pressure liquid chromatography techniques. Quantitative analyses of proteins which require 100% mass recovery of the protein are still beset with problems, especially when the total injected amount of protein decreases to below 10 micrograms. Stainless-steel frits have been cited for their deleterious effects on chromatography in general. In addition, the frits have specifically been found to be a significant contributor to irreversible protein loss--particularly when protein sample sizes are on the order of 1 microgram or less. The findings presented below should therefore be of concern to those using HPLC for protein work.
