Sadek P C, Carr P W, Bowers L D, Haddad L C
Anal Biochem. 1986 Mar;153(2):359-71. doi: 10.1016/0003-2697(86)90104-1.
A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amount and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.
本文报道了蛋白质在商用反相高效液相色谱填充材料上不可逆吸附的放射化学研究。研究条件与蛋白质高效液相色谱分离中使用的条件相似。报道了两种蛋白质，即卵清蛋白和细胞色素c的用量和接触时间的影响。其他结果包括用蛋白质进行柱预处理、硅醇亲和流动相封闭剂以及填充材料类型对不可逆吸附程度的影响。损失过程显示至少是双相的，并且不同蛋白质的损失机制不同。
