尼日利亚扎里亚阿哈穆杜·贝洛大学内冈比亚按蚊复合体中微孢子虫、恶性疟原虫和班氏吴策线虫可能共存情况的分析

Analysis of Possible Coexistence of Microsporidia, Plasmodium falciparum and Wuchereria bancrofti in Anopheles gambiae s.l within Ahmadu Bello University, Zaria, Nigeria.

作者信息

Aliyu Mukhtar, Salman Abdulmalik Abdullahi, Ibrahim Mohammed Auwal, Balogun Emmanuel Oluwadare, Shuaibu Mohammed Nasir

机构信息

Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.

African Centre of Excellence on Neglected Tropical Disease and Forensic Biotechnology, Ahmadu Bello University, Zaria, Nigeria.

出版信息

Acta Parasitol. 2025 Jan 24;70(1):23. doi: 10.1007/s11686-024-00971-3.

DOI:10.1007/s11686-024-00971-3

PMID:39853489

Abstract

PURPOSE

Anopheles gambiae is a vector of Plasmodium falciparum and Wuchereria bancrofti. Endosymbionts are reported to block development of various parasites in mosquitoes. Microsporidia was reported to affect the development of P. falciparum in mosquitoes. Data on such observation is limited in Nigeria.

METHODS

Therefore, the prevalence of Microsporidia and its coinfection with W. bancrofti and P. falciparum in An. gambiae s.l was studied within Ahmadu Bello University, Zaria.

RESULTS

Of the 912 mosquitoes sampled, 124 were An. gambiae s.l The midgut assessment of the Anopheles mosquitoes using light microscopy and polymerase chain reaction (PCR) showed a 12% prevalence of a mono microsporidia infection with no coinfection with either P. falciparum or W. bancrofti. Only 4.03% of the An. gambiae s.l. were found to be coinfected with P. falciparum and W. bancrofti while no mosquito harboured all the microorganisms CONCLUSION: This data further supports the potential of Microsporidia as an antagonist for the development of pathogens in mosquitoes.

摘要

目的

冈比亚按蚊是恶性疟原虫和班氏吴策线虫的传播媒介。据报道，内共生菌可阻止蚊子体内各种寄生虫的发育。据报道，微孢子虫会影响蚊子体内恶性疟原虫的发育。在尼日利亚，关于此类观察的数据有限。

方法

因此，在扎里亚的阿哈穆杜·贝洛大学内，研究了冈比亚按蚊复合体内微孢子虫的感染率及其与班氏吴策线虫和恶性疟原虫的混合感染情况。

结果

在采集的912只蚊子中，有124只是冈比亚按蚊复合体。使用光学显微镜和聚合酶链反应（PCR）对按蚊中肠进行评估，结果显示微孢子虫单一感染的感染率为12%，未与恶性疟原虫或班氏吴策线虫发生混合感染。仅4.03%的冈比亚按蚊复合体被发现同时感染了恶性疟原虫和班氏吴策线虫，而没有蚊子同时携带所有这些微生物。结论：该数据进一步支持了微孢子虫作为蚊子体内病原体发育拮抗剂的潜力。