Djebbara Sara, Belguendouz Houda, Soufli Imene, Hannachi Leila, Ameur Fahima, Benazzouz Sara, Benkhelifa Sarra, Terrahi Malika, Achour Karima, Amir Zine-Charaf, Amri Manel, Touil-Boukoffa Chafia
Cytokines and NO Synthases Team, LBCM, FSB, USTHB, BP 32 El Alia, Bab Ezzouar, Algiers, 16111, Algeria.
Department of Pathological Anatomy, University Hospital Center Mustapha Pacha, Algiers, Algeria.
Acta Parasitol. 2025 Jan 24;70(1):34. doi: 10.1007/s11686-024-00944-6.
Since extract of the laminated layer (LL) from E. granulosus showed immuno-modulatory effects in vitro and in vivo, we sought to determine its effect on the onset, development, and evolution of experimental auto-immune uveitis (EAU). The latter is a model of some human diseases with ocular inflammation that can cause blindness.
E. granulosus LL extract was either injected before EAU induction for the pretreated group or later for treated group. Ocular exploration was made by retinal histological and immunohistological (CD86, CD4, CD8) analysis. Myeloperoxidase (MPO), Superoxide dismutase (SOD), Catalase enzymatic activities (CAT), and Malondialdehyde (MDA), Nitric oxide (NO), Urea, and TNF-α levels were measured in plasma.
LL injection attenuated retinal histological damage and reduced cells infiltration. Also, LL decreased systemic inflammatory and oxidative markers as well as TNF-α production and increased antioxidant parameters.
Interestingly, we observed a protective effect of E. granulosus LL extract during EAU. LL appears to ameliorate retinal damage by down-regulating inflammatory responses. Our results support LL immunomodulatory effects during autoimmune diseases and offer a promising prospect for helminthic therapy during autoimmune uveitis.
由于细粒棘球绦虫层状层（LL）提取物在体外和体内均显示出免疫调节作用，我们试图确定其对实验性自身免疫性葡萄膜炎（EAU）的发病、发展和演变的影响。EAU是一些可导致失明的眼部炎症性人类疾病的模型。
细粒棘球绦虫LL提取物在EAU诱导前注射给预处理组，或在诱导后注射给治疗组。通过视网膜组织学和免疫组织学（CD86、CD4、CD8）分析进行眼部检查。测定血浆中的髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、过氧化氢酶活性（CAT）以及丙二醛（MDA）、一氧化氮（NO）、尿素和肿瘤坏死因子-α水平。
注射LL可减轻视网膜组织学损伤并减少细胞浸润。此外，LL降低了全身炎症和氧化标志物以及肿瘤坏死因子-α的产生，并增加了抗氧化参数。
有趣的是，我们观察到细粒棘球绦虫LL提取物在EAU期间具有保护作用。LL似乎通过下调炎症反应来改善视网膜损伤。我们的结果支持LL在自身免疫性疾病中的免疫调节作用，并为自身免疫性葡萄膜炎期间的蠕虫疗法提供了有前景的展望。
