Giorno R
Anat Rec. 1985 Jan;211(1):43-7. doi: 10.1002/ar.1092110108.
The distribution of the intermediate filament protein vimentin in peripheral lymphoid tissues was determined using a monoclonal antibody. Frozen sections of tissue were stained using an avidin-biotin immunoperoxidase method. The antibody stained endothelial cells in spleen, lymph node, and tonsil. Unusual rod-like structures were revealed in the sinusoid-lining cells of the spleen. A variety of reticulum cells was detected, including fibroblastic reticulum cells, histiocytic reticulum cells (tingible body macrophages), and splenic marginal zone macrophages. Very few lymphocytes were immunoreactive. Only weak cytoplasmic immunoreactivity was observed in lymphocytes of the periarteriolar lymphocyte sheath of the spleen. The monoclonal antibody employed appears to be of limited usefulness in detecting normal lymphocytes, but is strongly reactive with endothelial structures and some types of reticulum cells.
使用单克隆抗体确定中间丝蛋白波形蛋白在外周淋巴组织中的分布。组织冰冻切片采用抗生物素蛋白-生物素免疫过氧化物酶法染色。该抗体可对脾脏、淋巴结和扁桃体中的内皮细胞进行染色。在脾脏的血窦内衬细胞中发现了异常的杆状结构。检测到多种网状细胞，包括成纤维网状细胞、组织细胞性网状细胞（含铁血黄素巨噬细胞）和脾边缘区巨噬细胞。很少有淋巴细胞具有免疫反应性。在脾脏动脉周围淋巴细胞鞘的淋巴细胞中仅观察到微弱的细胞质免疫反应性。所使用的单克隆抗体在检测正常淋巴细胞方面似乎用途有限，但与内皮结构和某些类型的网状细胞有强烈反应。
