Lopez Guillaume L, Adda-Bouchard Yasmine, Laulhé Xavier, Chamberlain Gabriel, Bourguignon Léa, Charpentier Tania, Cyr Daniel G, Lamarre Alain
Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique (INRS), Laval, QC, Canada.
Centre Armand-Frappier Santé Biotechnologie, Institut national de la recherche scientifique (INRS), Laval, QC, Canada.
J Hazard Mater. 2025 May 5;488:137316. doi: 10.1016/j.jhazmat.2025.137316. Epub 2025 Jan 21.
The increasing prevalence of nanoplastics (NPs) in the environment, particularly polystyrene (PS) nanoparticles, raises concerns regarding their potential impact on human and animal health. Given their small size, NPs can cross biological barriers and accumulate in organs, including those critical for immune functions. This study investigates the effects of short-term oral exposure to 100 and 500 nm PS NPs on the adaptive immune responses during viral infections in vivo, using vesicular stomatitis virus (VSV) and lymphocytic choriomeningitis virus (LCMV) as models. Male and female C57BL/6 mice were orally exposed to PS NP for a period of 28 days, during which they were infected with either VSV or LCMV to study the humoral and cellular responses, respectively. The humoral responses were assessed by measuring total and VSV-specific antibody levels, and splenic immune populations. T cell phenotypes, activation, exhaustion and functionality towards LCMV epitopes were studied as readouts of the cellular responses. Our results demonstrate that short-term NP exposure does not significantly affect the generation or neutralizing capacity of antibodies against VSV, nor the cellular responses directed against LCMV. These findings indicate that, under these conditions, PS NP exposure does not significantly compromise the adaptive immune responses during viral infections, underscoring the value of in vivo models.
纳米塑料（NPs），尤其是聚苯乙烯（PS）纳米颗粒在环境中的日益普遍，引发了人们对其对人类和动物健康潜在影响的担忧。鉴于其尺寸小，纳米颗粒可以穿过生物屏障并在器官中积累，包括对免疫功能至关重要的器官。本研究以水泡性口炎病毒（VSV）和淋巴细胞性脉络丛脑膜炎病毒（LCMV）为模型，研究了短期口服100和500纳米的PS纳米颗粒对体内病毒感染期间适应性免疫反应的影响。雄性和雌性C57BL/6小鼠口服PS纳米颗粒28天，在此期间分别感染VSV或LCMV以研究体液和细胞反应。通过测量总抗体和VSV特异性抗体水平以及脾免疫细胞群来评估体液反应。研究了T细胞表型、对LCMV表位的激活、耗竭和功能，作为细胞反应的读数。我们的结果表明，短期接触纳米颗粒不会显著影响针对VSV的抗体的产生或中和能力，也不会显著影响针对LCMV的细胞反应。这些发现表明，在这些条件下，接触PS纳米颗粒不会显著损害病毒感染期间的适应性免疫反应，强调了体内模型的价值。
