Amin Omar M, Chaudhary Anshu, Caracciolo Makoto Enoki, Rubtsova Nataliya Yu, Wendt Camila, Lisitsyna Olga, Kuzmina Tetiana A, de Souza Wanderley, Singh Hridaya S
Institute of Parasitic Diseases, 11445 E. Via Linda, # 2-419, Scottsdale, AZ, 85259, USA.
Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut, Uttar Pradesh, 25004, India.
Syst Parasitol. 2025 Jan 24;102(1):16. doi: 10.1007/s11230-024-10212-w.
Corynosoma pseudohamanni Zdzitowiecki, 1984 (Polymorphidae) was described from the intestinal tract of 5 species of seals including the type and main host, the Weddell seal Leptonycotes weddellii (Lesson) in the South Shetlands, West Antarctica. Notothenia coriiceps was the primary paratenic host of 14 fish hosts reported in the original description. We describe excysted juveniles from the body cavity of the major paratenic host, Notothenia coriiceps Richardson collected off Galindez Island, Argentine Islands, West Antarctica for the first time. The original description was not "based on material collected from the final hosts (seals) and paratenic hosts (fish)" as stated since no description of juveniles was given then. Our excysted juveniles were generally smaller than reported adults and many of the other measurements were comparable to those of the adults in the original description. We added many of the incomplete measurements of adults in the original description especially those of the underdeveloped reproductive structures. We compared our morphometric description with the only other one available for juveniles collected from three paratenic fish hosts from Prince Gustav Channel, Antarctica. We have added informative optical microscopy images and SEM images of internal and external structures, previously missed in the original description. Energy Dispersive X-ray analysis revealed the highest levels of calcium in all hooks and hook roots compared to sulfur and phosphorus. We also provide a molecular characterization of the species for the first time. Newly generated sequences of the mitochondrial cytochrome c oxidase subunit 1 (mtcox1) gene from isolates of C. pseudohamanni cystacanths were compared with sequences of other acanthocephalans available in GenBank. Phylogenetic analyses based on maximum likelihood (ML) and Bayesian inference (BI) methods of the cox1 dataset placed all the species of Corynosoma in a single clade, with strong support. The mt cox1 sequences of C. pseudohamanni formed a strongly supported individual clade with the published sequences of Corynosoma nortmeri and Corynosoma magdaleni from the North Sea, Germany. We have determined the genetic diversity of C. pseudohamanni and compare it with other Corynosoma species haplotype diversity and distribution.
伪哈曼氏冠吻棘头虫（Corynosoma pseudohamanni）Zdzitowiecki，1984年（多形科）是从包括模式宿主和主要宿主在内的5种海豹的肠道中描述的，模式宿主和主要宿主为南极西部南设得兰群岛的威德尔海豹（Leptonycotes weddellii）（Lesson）。在最初的描述中，小头南极鱼（Notothenia coriiceps）是14种鱼类宿主的主要转续宿主。我们首次描述了从南极西部阿根廷群岛加林德斯岛附近采集的主要转续宿主小头南极鱼（Notothenia coriiceps Richardson）体腔中的脱囊幼虫。最初的描述并非如所述“基于从终末宿主（海豹）和转续宿主（鱼类）收集的材料”，因为当时并未给出幼虫的描述。我们的脱囊幼虫通常比报道的成虫小，并且许多其他测量值与最初描述中的成虫相当。我们补充了最初描述中许多成虫不完整的测量值，特别是未发育成熟的生殖结构的测量值。我们将我们的形态测量描述与从南极古斯塔夫王子海峡的三种转续鱼类宿主收集的幼虫的唯一其他可用描述进行了比较。我们添加了信息丰富的光学显微镜图像以及内部和外部结构的扫描电子显微镜图像，这些在最初的描述中均未出现。能量色散X射线分析显示，与硫和磷相比，所有钩和钩根中的钙含量最高。我们还首次提供了该物种的分子特征。将新生成的伪哈曼氏冠吻棘头虫囊尾蚴分离株的线粒体细胞色素c氧化酶亚基1（mtcox1）基因序列与GenBank中其他棘头虫的序列进行了比较。基于cox1数据集的最大似然（ML）和贝叶斯推断（BI）方法的系统发育分析将所有冠吻棘头虫物种置于一个单系分支中，支持度很高。伪哈曼氏冠吻棘头虫的mt cox1序列与来自德国北海的诺特氏冠吻棘头虫（Corynosoma nortmeri）和马格达莱纳冠吻棘头虫（Corynosoma magdaleni）的已发表序列形成了一个得到有力支持的单独分支。我们确定了伪哈曼氏冠吻棘头虫的遗传多样性，并将其与其他冠吻棘头虫物种的单倍型多样性和分布进行了比较。
