新型 microRNA miR-63 对珍珠贝 Pinctada fucata martensii 的免疫调节作用。
Immunomodulatory effects of one novel microRNA miR-63 in pearl oyster Pinctada fucata martensii.
机构信息
Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China.
Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China.
出版信息
Fish Shellfish Immunol. 2023 Sep;140:109002. doi: 10.1016/j.fsi.2023.109002. Epub 2023 Aug 15.
Novel microRNA miR-63 (novel-miR-63) from pearl oyster Pinctada fucata martensii (Pm-novel-miR-63) is a species-specific miRNA. Our previous research has shown that the expression of Pm-novel-miR-63 was significantly downregulated at 24 h after nucleus transplantation. In this study, we analyzed the function and regulatory role of Pm-novel-miR-63 in the immune response of pearl oysters. The results showed that Pm-novel-miR-63 expression increased after the stimulation of pathogen associated molecular patterns at 6-12 h, and the activity of immune and antioxidant enzymes in the serum decreased after Pm-novel-miR-63 overexpression. Transcriptome analysis revealed that Pm-novel-miR-63 participated in regulating transplantation immunity through the Notch and mRNA surveillance signaling pathways. Target prediction and dual luciferase analysis revealed that Pm-GDP-FucTP, Pm-CysLTR2, and Pm-RLR were the target genes of Pm-novel-miR-63. These results suggested that Pm-novel-miR-63 participated in regulating the immune response in pearl oysters and can serve as a new interference target to reasonably control excessive immune rejection in pearl culture.
来自马氏珠母贝（Pinctada fucata martensii）的新型 microRNA miR-63（novel-miR-63）是一种物种特异性 miRNA。我们之前的研究表明，在细胞核移植后 24 小时，Pm-novel-miR-63 的表达显著下调。在这项研究中，我们分析了 Pm-novel-miR-63 在珍珠贝免疫反应中的功能和调节作用。结果表明，病原体相关分子模式刺激后 6-12 小时，Pm-novel-miR-63 的表达增加，血清中免疫和抗氧化酶的活性在 Pm-novel-miR-63 过表达后降低。转录组分析表明，Pm-novel-miR-63 通过 Notch 和 mRNA 监测信号通路参与调节移植免疫。靶基因预测和双荧光素酶分析表明，Pm-GDP-FucTP、Pm-CysLTR2 和 Pm-RLR 是 Pm-novel-miR-63 的靶基因。这些结果表明，Pm-novel-miR-63 参与调节珍珠贝的免疫反应，可以作为新的干扰靶点，合理控制珍珠养殖中的过度免疫排斥。
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