Li Hongyan, Zong Ying, Chen Weijia, Zhao Yan, Geng Jianan, He Zhongmei, Du Rui
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China.
Jilin Provincial Engineering Research Center for Efficient Breeding and Product Development of Sika Deer, Jilin Agricultural University, Changchun 130118, China.
Foods. 2025 Jan 9;14(2):181. doi: 10.3390/foods14020181.
Deer oil (DO) is a potentially beneficial functional oil; however, its sensitivity to environmental factors (e.g., oxygen and heat), difficulty in transport, and unfavorable taste hinder practical use. In this study, DO was encapsulated through the cohesive action of soy protein isolate (SPI) and chitosan (CS). The optimal preparation conditions yielded microcapsules with DO's highest encapsulation efficiency (EE) (85.28 ± 1.308%) at an SPI/CS mixing ratio of 6:1 and a core-to-wall ratio of 1:2 at pH 6. Fluorescence and scanning electron microscopy were utilized to examine the microcapsules' structure, showing intact surfaces and effective encapsulation of oil droplets through SPI/CS composite coalescence. Through Fourier transform infrared spectroscopy (FTIR), the electrostatic interplay between SPI and CS was verified during the merging process. At room temperature, the microcapsules resisted core oxidation by reducing gas permeation. In vitro simulated digestion results indicated the microcapsules achieved a slow and sustained release of DO in the intestinal tract. This study further expands the application scope of deer oil and promotes the development of deer oil preparations and functional foods.
鹿油（DO）是一种具有潜在益处的功能油；然而，它对环境因素（如氧气和热）敏感、运输困难且味道不佳，阻碍了其实际应用。在本研究中，通过大豆分离蛋白（SPI）和壳聚糖（CS）的凝聚作用对鹿油进行了微胶囊化。最佳制备条件下，在SPI/CS混合比例为6:1、芯壁比为1:2、pH值为6时，得到了具有最高包封率（EE）（85.28±1.308%）的微胶囊。利用荧光和扫描电子显微镜检查微胶囊的结构，结果显示其表面完整，通过SPI/CS复合凝聚有效地包封了油滴。通过傅里叶变换红外光谱（FTIR），证实了合并过程中SPI和CS之间的静电相互作用。在室温下，微胶囊通过减少气体渗透来抵抗核心氧化。体外模拟消化结果表明，微胶囊在肠道中实现了鹿油的缓慢持续释放。本研究进一步拓展了鹿油的应用范围，推动了鹿油制剂和功能性食品的发展。
