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糖尿病和非糖尿病慢性肾脏病患者的T细胞亚群及分化偏向

T-Cell Subpopulations and Differentiation Bias in Diabetic and Non-Diabetic Patients with Chronic Kidney Disease.

作者信息

Granda Alacote Ana Cecilia, Goyoneche Linares Gabriela, Castañeda Torrico María Gracia, Diaz-Obregón Daysi Zulema, Núñez Michael Bryant Castro, Murillo Carrasco Alexis Germán, Liendo Cesar Liendo, Rufasto Goche Katherine Susan, Correa Víctor Arrunátegui, de León Delgado Joel

机构信息

Faculty of Natural Sciences and Mathematics, Universidad Nacional Federico Villarreal, Lima 15001, Peru.

ONG Innovation and Science for the Care and Support of Society-INNOVACARE, Lima 15036, Peru.

出版信息

Biomedicines. 2024 Dec 24;13(1):3. doi: 10.3390/biomedicines13010003.

DOI:10.3390/biomedicines13010003
PMID:39857588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11759818/
Abstract

BACKGROUND

Chronic kidney disease (CKD) patients often experience dysregulated inflammation, particularly when compounded by comorbidities such as type 2 diabetes (T2D).

OBJECTIVE

The aim of this study was to determine whether T2D influences the profile of memory T lymphocytes, regulatory T cells (Tregs), and the gene expression of transcription factors such as , , , and in CKD patients.

METHODS

Twenty-two CKD patients undergoing hemodialysis were selected for the study. Flow cytometry was used to identify naïve T cells, Tregs (CD4+CD25+CD127-), central memory T lymphocytes (CCR7+CD45RA-), effector memory T lymphocytes (CCR7-CD45RA-), and TEMRA cells (CCR7-CD45RA+). The expression of helper T cell differentiation regulatory genes was assessed using real-time RT-PCR.

RESULTS

Both helper and cytotoxic effector memory T cell populations were found to be higher than naïve lymphocytes in CKD patients, regardless of T2D status. However, Tregs were significantly more frequent in diabetic CKD patients (5.1 ± 2.6%) compared to non-diabetic patients (2.8 ± 3.1%). In terms of transcription factor expression, a significant correlation was observed between T-bet and in diabetic patients, and between RORyT and FOXP3 in non-diabetic patients.

CONCLUSIONS

While T2D does not notably alter the distribution of memory T cells in CKD patients, it significantly impacts the frequency of Tregs and their correlation with pro-inflammatory transcription factors like and .

摘要

背景

慢性肾脏病（CKD）患者常出现炎症调节异常，尤其是合并2型糖尿病（T2D）等合并症时。

目的

本研究旨在确定T2D是否会影响CKD患者记忆性T淋巴细胞、调节性T细胞（Tregs）的分布以及转录因子如 、 、 和 的基因表达。

方法

选择22例接受血液透析的CKD患者进行研究。采用流式细胞术鉴定初始T细胞、Tregs（CD4 + CD25 + CD127 - ）、中枢记忆性T淋巴细胞（CCR7 + CD45RA - ）、效应记忆性T淋巴细胞（CCR7 - CD45RA - ）和TEMRA细胞（CCR7 - CD45RA + ）。使用实时RT-PCR评估辅助性T细胞分化调节基因的表达。

结果

无论T2D状态如何，CKD患者中辅助性和细胞毒性效应记忆性T细胞群体均高于初始淋巴细胞。然而，与非糖尿病患者（2.8±3.1%）相比，糖尿病CKD患者中的Tregs明显更常见（5.1±2.6%）。在转录因子表达方面，糖尿病患者中T-bet与 之间以及非糖尿病患者中RORyT与FOXP3之间存在显著相关性。

结论

虽然T2D不会显著改变CKD患者记忆性T细胞的分布，但它会显著影响Tregs的频率及其与 和 等促炎转录因子的相关性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/7bd32c27380e/biomedicines-13-00003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/438c6f804aba/biomedicines-13-00003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/68aba1161f9c/biomedicines-13-00003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/8c139bab1a7b/biomedicines-13-00003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/23733ad673f1/biomedicines-13-00003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/7bd32c27380e/biomedicines-13-00003-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/438c6f804aba/biomedicines-13-00003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/68aba1161f9c/biomedicines-13-00003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/8c139bab1a7b/biomedicines-13-00003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/23733ad673f1/biomedicines-13-00003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/754b/11759818/7bd32c27380e/biomedicines-13-00003-g005.jpg

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